Construction of Fucoxanthin Vector Based on Binding of Whey Protein Isolate and Its Subsequent Complex Coacervation with Lysozyme

Journal of Agricultural and Food Chemistry
Junxiang ZhuDongfeng Wang

Abstract

In this study, a novel vector for fucoxanthin (FX) was constructed using the ligand-binding property of whey protein isolate and its subsequent heteroprotein complex coacervation with lysozyme. The results showed that FX could quench the intrinsic fluorescence of the whey protein isolate by a static mechanism, indicating that they could spontaneously form a nanocomplex through non-covalent interactions. Moreover, the structural and electrostatic properties of the resulting whey protein were different from those before the binding of FX, and this could be well explained by molecular dynamics simulation. The size and ζ-potential tests showed that when the whey protein isolate was combined with FX and then coacervated with lysozyme, the heteroprotein ratio and pH, which affect the coacervation process, also changed compared to those of the free whey protein isolate. FT-IR spectroscopy results showed that FX was successfully encapsulated into complex coacervates. In addition, the heteroprotein system exhibited a higher loading efficiency and also provided a better protection for FX in heating, storage, and simulated gastrointestinal environments.

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Citations

Oct 9, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ying BaiHang Qi
Dec 17, 2020·Comprehensive Reviews in Food Science and Food Safety·Chao WuMing Du
Aug 30, 2019·Journal of Agricultural and Food Chemistry·Junxiang ZhuQingjie Sun

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