Construction of gag-chimeric viruses between HIV-1 and SIVmac that are capable of productive multi-cycle infection

Microbes and Infection
Kazuya KamadaAkio Adachi

Abstract

Forty-nine recombinant viral clones between human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus from the rhesus monkey (SIVmac), which carry chimeric gag (capsid/p2 region) genes in the background of the HIV-1 genome, were constructed to establish an HIV-1/monkey infection model system for human AIDS. Upon transfection, all the recombinants generated progeny virions at a level comparable to the parental HIV-1 clone and no major abnormalities were found in the virions, as examined by Western blot analysis. In infection experiments, 18 recombinants grew in human lymphocytic cells and six of these clones propagated as well as the parental virus, as monitored by virion associated-reverse transcriptase production. By contrast, none of the recombinants grew at a detectable level in monkey lymphocytic cells. The defective replication site(s) in human cells for non-infectious recombinants was mapped to the step before and/or during reverse transcription. Our results described here showed that HIV-1 type chimeric viruses between HIV-1 and SIVmac, which are capable of spreading productive infection, are readily constructed throughout the capsid/p2 region. In addition, it is suggested that there may be a viral de...Continue Reading

References

Nov 1, 1992·The Journal of General Virology·S SakuragiM Hayami
Mar 1, 1992·AIDS Research and Human Retroviruses·R Shibata, A Adachi
Aug 29, 1995·Proceedings of the National Academy of Sciences of the United States of America·K UberlaB Oberg
Nov 1, 1995·The Journal of General Virology·R ShibataA Adachi
Aug 15, 1998·Biochemical and Biophysical Research Communications·M KawamuraA Adachi
Sep 24, 1999·Biochemical and Biophysical Research Communications·H AkariA Adachi
May 14, 2004·Journal of Virology·Theodora HatziioannouPaul D Bieniasz
Oct 14, 2004·Journal of Virology·Yasuhiro IkedaGreg J Towers

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