Construction of genotyping-by-sequencing based high-density genetic maps and QTL mapping for fusarium wilt resistance in pigeonpea

Scientific Reports
Rachit K SaxenaRajeev K Varshney

Abstract

Fusarium wilt (FW) is one of the most important biotic stresses causing yield losses in pigeonpea. Genetic improvement of pigeonpea through genomics-assisted breeding (GAB) is an economically feasible option for the development of high yielding FW resistant genotypes. In this context, two recombinant inbred lines (RILs) (ICPB 2049 × ICPL 99050 designated as PRIL_A and ICPL 20096 × ICPL 332 designated as PRIL_B) and one F2 (ICPL 85063 × ICPL 87119) populations were used for the development of high density genetic maps. Genotyping-by-sequencing (GBS) approach was used to identify and genotype SNPs in three mapping populations. As a result, three high density genetic maps with 964, 1101 and 557 SNPs with an average marker distance of 1.16, 0.84 and 2.60 cM were developed in PRIL_A, PRIL_B and F2, respectively. Based on the multi-location and multi-year phenotypic data of FW resistance a total of 14 quantitative trait loci (QTLs) including six major QTLs explaining >10% phenotypic variance explained (PVE) were identified. Comparative analysis across the populations has revealed three important QTLs (qFW11.1, qFW11.2 and qFW11.3) with upto 56.45% PVE for FW resistance. This is the first report of QTL mapping for FW resistance in pig...Continue Reading

References

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Nov 15, 2015·Plant Science : an International Journal of Experimental Plant Biology·Rajeev K Varshney

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Citations

Apr 1, 2018·Plant, Cell & Environment·Mahsa Mousavi-DerazmahallehDavid Edwards
May 1, 2018·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Rachit K SaxenaRajeev K Varshney
Jan 12, 2020·Plant Biotechnology Journal·Pallavi SinhaRajeev K Varshney
Dec 18, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Rachit K SaxenaRajeev K Varshney
Feb 18, 2020·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Abhishek BohraRachit K Saxena
Jun 8, 2018·Breeding Science·Kul Bhushan SaxenaRajeev K Varshney
Dec 19, 2018·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Rajeev K VarshneyRachit K Saxena
Dec 4, 2019·Plant Breeding = Zeitschrift Für Pflanzenzüchtung·Chris OjiewoRajeev K Varshney
Apr 5, 2018·Frontiers in Plant Science·Francy L García-AriasVictor M Núñez Zarantes
Oct 21, 2020·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Rachit K SaxenaRajeev K Varshney
Nov 17, 2020·Frontiers in Genetics·Uday Chand JhaSwarup Kumar Parida
Dec 23, 2020·International Journal of Molecular Sciences·Sandeep Kaur DhaliwalPrashant Kaushik

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Methods Mentioned

BETA
PCR
genotyping
dissection

Software Mentioned

Biomercator
Seq
TASSEL
BSA
WinQTLCart2
Burrows
MapChart
GAB
GBS
- GBS

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