Construction of Ginsenoside Nanoparticles with pH/Reduction Dual Response for Enhancement of Their Cytotoxicity Toward HepG2 Cells

Journal of Agricultural and Food Chemistry
Yu XuJiang-Ning Hu

Abstract

The aim of this study is to construct a pH- and reduction-responsive nanodrug delivery system to effectively deliver a ginsenoside (Rh2) and enhance its cytotoxicity against human hepatocarcinoma cells (HepG2). Here, pullulan polysaccharide was grafted by urocanic acid and α-lipoic acid (α-LA) to obtain a copolymer, α-LA-conjugated N-urocanyl pullulan (LA-URPA), which was expected to have pH and redox dual response. Then, the copolymer LA-URPA was used to encapsulate ginsenoside Rh2 to form Rh2 nanoparticles (Rh2 NPs). The results showed that Rh2 NPs exhibited an average size of 119.87 nm with a uniform spherical morphology. Of note, Rh2 NPs showed a high encapsulation efficiency of 86.00%. Moreover, Rh2 NPs possessed excellent pH/reduction dual-responsive drug release under acidic conditions (pH 5.5) and glutathione (GSH) stimulation with a low drug leakage of 14.8% within 96 h. Furthermore, Rh2 NPs with pH/reduction dual response had higher cytotoxicity than Rh2 after incubation with HepG2 cells for 72 h, indicating that Rh2 NPs had a longer circulation time. After the treatment with Rh2 NPs, the excessive increase of reactive oxygen species and the decrease of superoxide dismutase, glutathione (GSH), and mitochondrial membra...Continue Reading

References

Feb 22, 2001·Journal of Controlled Release : Official Journal of the Controlled Release Society·Y KaneoY Yamaguchi
Apr 3, 2003·Nature Reviews. Cancer·Mace L RothenbergDavid H Johnson
Jun 16, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Yi GuYang Sai
Sep 30, 2009·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Houfu LiuChuan Li
Feb 12, 2014·The American Journal of Gastroenterology·Sean F AltekruseKatherine A McGlynn
May 6, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Kinam Park
Feb 24, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Anisha A D'Souza, Padma V Devarajan
Mar 2, 2016·Colloids and Surfaces. B, Biointerfaces·Maqusood AhamedAws Alshamsan
Jul 30, 2016·Acta Pharmaceutica Sinica. B·Xinli ChenChen Jiang
Dec 14, 2016·Toxicon : Official Journal of the International Society on Toxinology·Nikita DevnarainAnil A Chuturgoon
Dec 19, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Mengrui LiuGuangxi Zhai
Dec 2, 2017·The Journal of Biological Chemistry·Ashley Solmonson, Ralph J DeBerardinis
Feb 8, 2018·Nanomedicine : Nanotechnology, Biology, and Medicine·Liping HuangChunmeng Sun
Mar 17, 2018·Behavioural Brain Research·Cong LuXinmin Liu
Jul 10, 2018·Journal of Ginseng Research·Akash AhujaJae Youl Cho
Jul 10, 2018·Journal of Ginseng Research·Hyeongmin KimJaehwi Lee
Mar 8, 2019·International Journal of Biological Macromolecules·Qian RenJiaoning Tang
May 24, 2019·Nanomedicine : Nanotechnology, Biology, and Medicine·Mengjiao ZhouXiujuan Zhang
Aug 17, 2019·Advanced Drug Delivery Reviews·Siddharth PatelGaurav Sahay
Nov 26, 2019·Materials Science & Engineering. C, Materials for Biological Applications· SaurajYuvraj Singh Negi
Dec 10, 2019·International Journal of Pharmaceutics·Zengjun FangXiaoqing Cai
Mar 21, 2020·Saudi Pharmaceutical Journal : SPJ : the Official Publication of the Saudi Pharmaceutical Society·Mosa Alsehli
Jan 21, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Lin DaiJiandu Lei

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