Construction of improved vectors for protein production in Pseudomonas aeruginosa

Gene
A A WatsonJohn S Mattick

Abstract

We report the construction of two cloning vectors that are based on the Pseudomonas-Escherichia shuttle vector, pUCP19. The new vectors, pUCPKS and pUCPSK, contain a significantly expanded multiple cloning site (MCS) with an adjacent T7 promoter sequence. In conjunction with specifically engineered host strains encoding an inducible T7 RNA polymerase, these vectors allow the controlled production of plasmid-encoded proteins in both Escherichia coli and Pseudomonas aeruginosa to analyse the spectrum of products encoded by cloned segments of DNA. The usefulness of these vectors was demonstrated by expressing the chloramphenicol acetyltransferase (CAT)-encoding gene.

References

Oct 18, 2001·Current Opinion in Biotechnology·H P Schweizer
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·Cynthia B WhitchurchJohn S Mattick
Sep 19, 2006·Journal of Bacteriology·Thomas S Murray, Barbara I Kazmierczak
Dec 11, 2007·Journal of Bacteriology·Thomas S Murray, Barbara I Kazmierczak
Jan 3, 2001·Journal of Bacteriology·A UrbanK E Jaeger
Feb 24, 2001·Journal of Bacteriology·Cornelia ReimmannDieter Haas
Jun 12, 2002·Journal of Bacteriology·Scott A BeatsonJohn S Mattick
Jun 12, 2002·Journal of Bacteriology·Scott A BeatsonJohn S Mattick
Jun 19, 2004·Journal of Bacteriology·Bixing HuangJohn S Mattick
Jan 20, 2004·Journal of Bacteriology·Pierre-Marie AkochyJacques Lapointe
Dec 17, 2004·Journal of Bacteriology·Petra Meier, Wilfried Wackernagel
Dec 22, 2009·BMC Microbiology·Tanja PiekarskiDieter Jahn
Apr 1, 2014·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Alessandro MaspoliCornelia Reimmann
Oct 2, 2002·Molecular Microbiology·Wilhelmina M HustonAlastair G McEwan
Nov 29, 2007·Cellular Microbiology·Laeticia AlibaudPierre Cosson
May 9, 2006·Molecular Microbiology·Barbara I KazmierczakThomas S Murray
Oct 26, 2005·Journal of Bioscience and Bioengineering·Ken OmoriTakuya Nihira
Jun 21, 2014·Environmental Microbiology·Olivier CunrathIsabelle J Schalk
Sep 12, 2000·Chemistry & Biology·K LiebetonK E Jaeger
Mar 22, 2008·FEMS Microbiology Letters·Sascha HausmannFrank Rosenau
Apr 11, 2003·Microbiology·Cecilia BerndtWilfried Wackernagel
Sep 17, 2011·Journal of Medical Microbiology·Tim C R ConibearHua Zhu

Citations

Oct 11, 1988·Nucleic Acids Research·S E West, B H Iglewski

Related Concepts

Escherichia
Pseudomonas Infections
Shuttle Vectors
Pseudomonas aeruginosa (antigen)
Alkalescens-Dispar Group
DNA Sequence - Cloning Site
Promoter
Pseudomonas aeruginosa
Escherichia Infection
Chloramphenicol O-Acetyltransferase

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Sexual Dimorphism in Neurodegeneration

There exist sex differences in neurodevelopmental and neurodegenerative disorders. For instance, multiple sclerosis is more common in women, whereas Parkinson’s disease is more common in men. Here is the latest research on sexual dimorphism in neurodegeneration

HLA Genetic Variation

HLA genetic variation has been found to confer risk for a wide variety of diseases. Identifying these associations and understanding their molecular mechanisms is ongoing and holds promise for the development of therapeutics. Find the latest research on HLA genetic variation here.

Super-resolution Microscopy

Super-resolution microscopy is the term commonly given to fluorescence microscopy techniques with resolutions that are not limited by the diffraction of light. Here are the latest discoveries pertaining to super-resolution microscopy.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Brain Lower Grade Glioma

Low grade gliomas in the brain form from oligodendrocytes and astrocytes and are the slowest-growing glioma in adults. Discover the latest research on these brain tumors here.

CD4/CD8 Signaling

Cluster of differentiation 4 and 8 (CD8 and CD8) are glycoproteins founds on the surface of immune cells. Here is the latest research on their role in cell signaling pathways.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.