Construction of improved vectors for protein production in Pseudomonas aeruginosa

A A WatsonJohn S Mattick


We report the construction of two cloning vectors that are based on the Pseudomonas-Escherichia shuttle vector, pUCP19. The new vectors, pUCPKS and pUCPSK, contain a significantly expanded multiple cloning site (MCS) with an adjacent T7 promoter sequence. In conjunction with specifically engineered host strains encoding an inducible T7 RNA polymerase, these vectors allow the controlled production of plasmid-encoded proteins in both Escherichia coli and Pseudomonas aeruginosa to analyse the spectrum of products encoded by cloned segments of DNA. The usefulness of these vectors was demonstrated by expressing the chloramphenicol acetyltransferase (CAT)-encoding gene.


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Related Concepts

Pseudomonas Infections
Shuttle Vectors
Pseudomonas aeruginosa (antigen)
Alkalescens-Dispar Group
DNA Sequence - Cloning Site
Pseudomonas aeruginosa
Escherichia Infection
Chloramphenicol O-Acetyltransferase

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