Construction of lacZ promoter probe vectors for use in Synechococcus: application to the identification of CO2-regulated promoters

Gene
D J ScanlanN G Carr

Abstract

It was shown that the Escherichia coli lacZ gene could be expressed in the cyanobacterium Synechococcus R2 PCC7942 both as a plasmid-borne form and also integrated into the chromosome. A promoterless form of the lacZ gene was constructed and used as a reporter gene to make transcriptional fusions with cyanobacterial promoters using a shuttle vector system and also via a process of integration by homologous recombination. Synechococcus R2 promoter-lacZ gene fusions were then used to identify CO2-regulated promoters, by quantitatively assessing beta-galactosidase activity under high and low CO2 conditions using a fluorescence assay. Several promoters induced under low CO2 conditions were detected.

References

Jun 1, 1986·Molecular & General Genetics : MGG·D Friedberg, J Seijffers
Jun 1, 1981·Analytical Biochemistry·D S Holmes, M Quigley
Apr 1, 1980·Journal of Molecular Biology·M J Casadaban, S N Cohen
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·L J ShimketsD Kaiser
Oct 1, 1986·Plant Physiology·Y MarcusA Kaplan

❮ Previous
Next ❯

Citations

Aug 7, 1999·FEMS Microbiology Letters·D L CarbonelliJ Zorzópulos
Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·C P WolkJ M Panoff
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·C ThelwellJ S Turner-Cavet
Nov 18, 2004·Journal of Bacteriology·David E WhitworthDavid A Hodgson
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Aaron Kaplan, Leonora Reinhold
Jul 17, 2003·Molecular Microbiology·Susana CampoyJordi Barbé
Dec 2, 2014·Applied Microbiology and Biotechnology·Kangming ChenRuanbao Zhou
Jan 1, 1992·Virchows Archiv. A, Pathological Anatomy and Histopathology·K DonathH J Günzl
May 1, 1995·Journal of Bacteriology·D J ScanlanN G Carr

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.