Construction of molecularly imprinted nanoparticles by employing ultrasound waves for selective determination of doxepin from human plasma samples: Modeling and optimization

Biomedical Chromatography : BMC
Sonia BahraniMaryam Arabi

Abstract

In this work, molecularly imprinted nanoparticles (MINPs) were applied as selective adsorbent for ultrasound-assisted micro-solid-phase extraction (UAMSPE) of doxepin (DP) from human plasma samples, which was then cleaned up, pre-concentrated and subjected to HPLC. The MINPs were synthesized based on a non-covalent approach by precipitation polymerization utilizing methacrylic acid and styrene as functional monomers, DP as template, ethylene glycol dimethacrylate as cross-linker and 2,2-azobisisobutyronitrile (AIBN) as initiator. The obtained MINPs were characterized by Fourier transform-infrared and field emission scanning electron microscopy. Factors influencing the efficiency of UAMSPE such as sonication time, volume of eluent solvent and amount of sorbent were investigated using a central composite design and the optimal points were identified as 4 min of sonication time, 380 μL of eluent solvent and 30 mg of sorbent. Under optimized conditions, the proposed method has linear responses in the range of 0.2-2000 ng mL-1 , with a satisfactory limit of detection of 0.04 ng mL-1 and limit of quantification of 0.11 ng mL-1 .

References

Jul 31, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Christine FrahnertKatja Grasmäder
May 9, 2006·International Journal of Biological Macromolecules·P B KandagalD H Manjunatha
Aug 10, 2007·Expert Opinion on Investigational Drugs·Haramandeep Singh, Philip M Becker
Aug 30, 2008·The Gerontologist·Sara Imhof, Brian Kaskie
Nov 26, 2008·Journal of Medical Toxicology : Official Journal of the American College of Medical Toxicology·Nicole WhiteCathleen Clancy
Jul 28, 2010·CNS Drugs·Juliane WeberPaul L McCormack
Jan 23, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ling-Hui ShengHong-Xun Zhang
Dec 3, 2014·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Mingqiong ZhouAiping Li
Oct 30, 2016·Therapeutic Delivery·Flavia LaffleurDuangratana Shuwisitkul
Oct 28, 2017·British Dental Journal·N Patel
Apr 28, 2018·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Sonia BahraniAli Daneshfar
Nov 6, 2018·Ultrasonics Sonochemistry·S WangJ W Kang
Nov 14, 2018·Ultrasonics Sonochemistry·Buxuan LiMin Chen
May 16, 2019·Advanced Materials·Huiqi Zhang

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