Construction of mouse A9 clones containing a single human chromosome tagged with neomycin-resistance gene via microcell fusion

Japanese Journal of Cancer Research : Gann
M KoiM Oshimura

Abstract

Normal human fibroblasts (MRC-5 or NTI-4) were transfected with pSV2-neo plasmid DNA. Fifty G418-resistant fibroblast clones were isolated and independently fused to mouse A9 cells. The cell hybrids were selected and isolated in the medium containing G418 plus ouabain. Since micronuclei were more efficiently induced in these hybrids compared to parental human fibroblasts by colcemid treatment, the transfer of neo-tagged human chromosomes in the hybrids to mouse A9 cells was performed via microcell fusion. Two hundred A9 microcell hybrids were isolated and karyotyped. Among them, thirteen microcell clones, each containing a single human chromosome 1, 2, 5, 6, 7, 8, 10, 11, 12, 15, 18, 19 or 20 were established. Isozyme analyses conformed the presence of each human chromosome in these A9 microcell clones. The results of Southern blot and chromosomal in situ hybridization analyses indicate that the human chromosomes in these clones were tagged with pSV2-neo plasmid DNA.

References

Jan 1, 1979·Journal of Supramolecular Structure·J W Shay, M A Clark
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M Koi, J C Barrett
Jan 1, 1985·Molecular and Cellular Biology·P J SaxonE J Stanbridge
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M E HarperG F Saunders

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Citations

Nov 1, 1992·Somatic Cell and Molecular Genetics·E Stubblefield, M Pershouse
Sep 1, 1990·Somatic Cell and Molecular Genetics·T S SidénD Röhme
Sep 1, 1993·Somatic Cell and Molecular Genetics·N OheY Fujii-Kuriyama
Feb 1, 1997·Mammalian Genome : Official Journal of the International Mammalian Genome Society·A SabileP Avner
Jul 25, 2008·Chromosoma·Karen J MeaburnJoanna M Bridger
Mar 16, 2013·Cytotechnology·Narumi UnoMitsuo Oshimura
Apr 1, 1995·Critical Reviews in Oncology/hematology·M MiyakiM Konishi
Jul 24, 1998·Mutation Research·K R TindallT A Kunkel
Jul 14, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Yasuhiro Kazuki, Mitsuo Oshimura
Mar 11, 1992·Proceedings of the National Academy of Sciences of the United States of America·W E LohB E Weissman
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·J D SipleyC W Anderson
May 17, 2000·The Journal of Experimental Medicine·J JankeS Scherneck
May 10, 2002·Nucleic Acids Research·Barbara C GodthelpMassgorzata Z Zdzienicka
Jan 5, 2002·Molecular and Cellular Biology·Maria Kraakman-van der ZwetMalgorzata Z Zdzienicka
Apr 6, 2013·Nucleic Acids Research·Artem V KononenkoVladimir Larionov
Aug 22, 2012·Cellular and Molecular Life Sciences : CMLS·Natalay KouprinaVladimir Larionov
Jan 6, 2016·Cancers·Hiroyuki KugohMitsuo Oshimura
Feb 1, 2014·Expert Opinion on Drug Delivery·Natalay KouprinaVladimir Larionov
May 1, 1995·Genes, Chromosomes & Cancer·M E Shaw, M A Knowles
Feb 7, 2008·Reproductive Biomedicine Online·Mitsuo Oshimura, Motonobu Katoh

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