Construction of Multi-Potent MicroRNA Sponge and Its Functional Evaluation

Methods in Molecular Biology
Suhwan Chang

Abstract

MicroRNA is a small, endogenous RNA that inhibits specific gene expression by interacting mostly on the UTR region of target mRNA. Among various methods to inhibit the microRNA, microRNA sponge is a construct designed to inhibit specific microRNA by providing excess amount of target mRNA. Here, we describe a method to generate multi-potent miRNA sponge which can inhibit multiple microRNAs simultaneously. In addition, two methods to examine the functionality of miRNA sponge will be introduced. This tool can be used to stably inhibit multiple microRNAs either in cell or in vivo, expanding the scope of functional analysis of specific microRNAs.

Citations

Apr 10, 2019·International Journal of Endocrinology·Xi SunXianghang Luo
Oct 12, 2019·Clinica Chimica Acta; International Journal of Clinical Chemistry·Dandan LiJinming Li
Jun 20, 2021·Nature Reviews. Drug Discovery·Melanie WinkleGeorge A Calin

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