Construction of Mycobacterium tuberculosis cdd knockout and evaluation of invasion and growth in macrophages

Memórias do Instituto Oswaldo Cruz
Anne Drumond VillelaDiógenes S Santos

Abstract

Cytidine deaminase (MtCDA), encoded by cdd gene (Rv3315c), is the only enzyme identified in nucleotide biosynthesis pathway of Mycobacterium tuberculosis that is able to recycle cytidine and deoxycytidine. An M. tuberculosis knockout strain for cdd gene was obtained by allelic replacement. Evaluation of mRNA expression validated cdd deletion and showed the absence of polar effect. MudPIT LC-MS/MS data indicated thymidine phosphorylase expression was decreased in knockout and complemented strains. The cdd disruption does not affect M. tuberculosis growth both in Mid- dlebrook 7H9 and in RAW 264.7 cells, which indicates that cdd is not important for macrophage invasion and virulence.

References

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Feb 23, 2017·Memórias do Instituto Oswaldo Cruz·Anne Drumond VillelaDiógenes Santiago Santos

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Citations

Dec 24, 2021·Microbiology Spectrum·Mario Alejandro Duque-VillegasCristiano Valim Bizarro

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Methods Mentioned

BETA
PCRs
electrophoresis
PCR

Software Mentioned

MudPIT
PatternLab
Comet
GraphPad Prism
Search Engine Processor ( SEPro )

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