Construction of Nanodroplet/Adiposome and Artificial Lipid Droplets

ACS Nano
Yang WangPingsheng Liu

Abstract

The lipid droplet (LD) is a cellular organelle that consists of a neutral lipid core with a monolayer-phospholipid membrane and associated proteins. Recent LD studies demonstrate its importance in metabolic diseases and biofuel development. However, the mechanisms governing its formation and dynamics remain elusive. Therefore, we developed an in vitro system to facilitate the elucidation of these mechanisms. We generated sphere-shaped structures with a neutral lipid core and a monolayer-phospholipid membrane by mechanically mixing neutral lipids and phospholipids followed by a two-step purification. We named the nanodroplet "adiposome". We then recruited LD structure-like/resident proteins to the adiposome, including the bacterial MLDS, Caenorhabditis elegans MDT-28/PLIN-1, or mammalian perilipin-2. In addition, adipose triglyceride lipase (ATGL) and apolipoprotein A1 (apo A-I) were recruited to adiposome. We termed the functional protein-coated adiposomes, Artificial Lipid Droplets (ALDs). With this experimental system, different proteins can be recruited to build ALDs for some biological goals and potential usage in drug delivery.

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Citations

Sep 16, 2017·Protein & Cell·Congyan Zhang, Pingsheng Liu
Jun 17, 2020·Journal of Cellular and Molecular Medicine·Lumin ChenWei-Chun Chang
Sep 15, 2018·Proteomics·Congyan Zhang, Pingsheng Liu
Apr 20, 2021·Free Radical Research·Mike LangeMaria Fedorova
May 3, 2021·Biochimica Et Biophysica Acta. Biomembranes·Jeffrey A JulienKerney Jebrell Glover
Jul 10, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Valerija VezočnikPeter Maček

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