Construction of neocentromere-based human minichromosomes by telomere-associated chromosomal truncation

Proceedings of the National Academy of Sciences of the United States of America
Richard SafferyK H Choo

Abstract

Neocentromeres (NCs) are fully functional centromeres that arise ectopically in noncentromeric regions lacking alpha-satellite DNA. Using telomere-associated chromosome truncation, we have produced a series of minichromosomes (MiCs) from a mardel(10) marker chromosome containing a previously characterized human NC. These MiCs range in size from approximately 0.7 to 1.8 Mb and contain single-copy intact genomic DNA from the 10q25 region. Two of these NC-based Mi-Cs (NC-MiCs) appear circular whereas one is linear. All demonstrate stability in both structure and mitotic transmission in the absence of drug selection. Presence of a functional NC is shown by binding a host of key centromere-associated proteins. These NC-MiCs provide direct evidence for mitotic segregation function of the NC DNA and represent examples of stable mammalian MiCs lacking centromeric repeats.

References

Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·C FarrH Cooke
Dec 1, 1993·European Journal of Biochemistry·A C Porter, J E Itzhaki
Aug 1, 1994·Human Molecular Genetics·S S TaylorC T Smith
Apr 1, 1994·Molecular and Cellular Biology·C E DabrowskiP A Schaffer
Jun 1, 1996·Molecular and Cellular Biology·S E HoltJ W Shay
Jul 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·R HellerW R Brown
May 1, 1996·Human Molecular Genetics·P M LansdorpH J Tanke
Apr 1, 1996·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·J KeresöG Hadlaczky
Feb 20, 1997·Nature·B van Steensel, T de Lange
Feb 1, 1996·Methods : a Companion to Methods in Enzymology·A M Killary, S T Lott
Aug 1, 1997·Human Molecular Genetics·M H ShenW Brown
Sep 23, 1997·The Journal of Cell Biology·D A StarrM L Goldberg
Dec 18, 1997·American Journal of Human Genetics·K H Choo
Jan 13, 1998·Nature Genetics·B C WilliamsG H Karpen
Mar 14, 1998·Proceedings of the National Academy of Sciences of the United States of America·P KalitsisK H Choo
May 21, 1998·Nature Biotechnology·M IkenoH Masumoto
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·K A HenningM A Ashlock
Jan 23, 1999·Chromosoma·H MasumotoN Suzuki
Apr 29, 1999·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·H TeleniusG Hadlaczky
May 14, 1999·Current Opinion in Genetics & Development·K W DobieG H Karpen
Jul 17, 1999·British Journal of Cancer·C N ParrisR F Newbold
Aug 26, 1999·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·R SafferyK H Choo
Apr 8, 2000·Trends in Cell Biology·K H Choo
May 10, 2000·Current Opinion in Cell Biology·A L Pidoux, R C Allshire
Jun 7, 2000·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·D O CoJ I Drayer
Aug 1, 2000·Human Molecular Genetics·J W YangW R Brown

❮ Previous
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Citations

Feb 6, 2002·The Journal of Gene Medicine·Richard Saffery, K H Andy Choo
Sep 1, 2005·Chromosoma·Brenda R Grimes, Zoia Larin Monaco
Nov 19, 2008·Chromosoma·Evgueni V AnanievScott Tingey
Feb 11, 2005·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Danielle V IrvineRichard Saffery
Jul 18, 2012·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Karen E Hayden
Jun 5, 2002·Trends in Genetics : TIG·Zoia Larin, José E Mejía
Feb 6, 2003·Gene·H J LippsJ Bode
May 30, 2001·Trends in Molecular Medicine·K H Choo
Jul 14, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Yasuhiro Kazuki, Mitsuo Oshimura
Mar 12, 2005·Nature Reviews. Genetics·Dominic J GloverDavid A Jans
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·F Lyn ChanLee H Wong
Dec 4, 2002·The Journal of Cell Biology·Jun-ichirou OhzekiHiroshi Masumoto
Apr 12, 2002·Cloning and Stem Cells·H Cooke
Mar 9, 2005·Cytogenetic and Genome Research·A KatoJ A Birchler
May 30, 2006·Proceedings of the National Academy of Sciences of the United States of America·Paul KalitsisK H Andy Choo
May 10, 2001·Proceedings of the National Academy of Sciences of the United States of America·H F Willard
Nov 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·Weichang YuJames A Birchler
Apr 26, 2008·FEBS Letters·P VagnarelliW C Earnshaw
Aug 31, 2006·Expert Opinion on Emerging Drugs·Matthieu D Lavigne, Dariusz C Górecki
Mar 11, 2005·Expert Opinion on Biological Therapy·Bruce A BunnellCarl F Perez
Oct 10, 2006·Pediatric Clinics of North America·Joydeep Basu, Huntington F Willard
Feb 7, 2008·American Journal of Human Genetics·Owen J MarshallK H Andy Choo
Aug 28, 2002·American Journal of Human Genetics·David J Amor, K H Andy Choo
Oct 26, 2005·Trends in Biotechnology·Danielle V IrvineRichard Saffery
May 11, 2005·Trends in Molecular Medicine·Joydeep Basu, Huntington F Willard
Nov 13, 2001·Developmental Cell·K H Choo
Feb 16, 2015·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Jun-ichirou OhzekiHiroshi Masumoto
Jan 19, 2015·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Robert L Katona
Nov 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Leslie A Mitchell, Jef D Boeke
Nov 24, 2004·The Journal of Biological Chemistry·Lee H WongK H Andy Choo
May 29, 2002·Gene Therapy·B R GrimesC J Farr
Jan 25, 2012·The Plant Journal : for Cell and Molecular Biology·Chunhui XuWeichang Yu
Dec 25, 2004·Infection and Immunity·Ryan M KellyStuart M Levitz
Jun 23, 2006·Infection and Immunity·Karen L WozniakStuart M Levitz
Nov 3, 2004·The Journal of Gene Medicine·Yun Chau LongOi Lian Kon

❮ Previous
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