Construction of non-toxic Escherichia coli and Vibrio cholerae strains expressing high and immunogenic levels of enterotoxigenic E. coli colonization factor I fimbriae

Vaccine
J TobiasAnn-Mari Svennerholm

Abstract

To express high quantities of colonization factor antigen I (CFA/I) derived from enterotoxigenic Escherichia coli (ETEC) for use in ETEC vaccines, the entire CFA/I operon consisting of four genes (cfa-A, -B, -C, -E) was cloned into plasmid expression vectors that could be maintained either with or without antibiotic selection. Expression from the powerful tac promoter was under the control of the lacIq repressor present on the plasmids. Fimbriae were expressed on the surface of both a non-toxigenic E. coli K12 strain and a non-toxigenic strain of Vibrio cholerae following induction with isopropyl-beta-D-thiogalactopyranoside (IPTG). It was found that the recombinant E. coli strains expressed up to 16-fold higher levels of CFA/I fimbriae compared to a reference strain which had previously been shown to be among the highest natural producers of the CFA/I fimbriae among tested wild type ETEC strains. Oral immunization with formalin-killed recombinant E. coli bacteria over-expressing CFA/I induced significantly higher serum IgA and IgG+M antibodies responses compared to the reference strain. Oral immunization with formalin-killed recombinant V. cholerae bacteria also induce strong CFA/I-specific serum IgA and IgG+M responses. We co...Continue Reading

References

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Citations

May 4, 2012·Expert Review of Vaccines·Ann-Mari Svennerholm, Anna Lundgren
Aug 10, 2012·Expert Review of Vaccines·Weiping Zhang, David A Sack
Jul 31, 2008·Expert Review of Vaccines·Ann-Mari Svennerholm, Joshua Tobias
Mar 31, 2015·Advanced Drug Delivery Reviews·Christopher J H Davitt, Ed C Lavelle
Nov 24, 2012·Microbiology·Manvendra SaxenaPeter M Smooker
May 10, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Christopher J H DavittEd C Lavelle
Aug 4, 2016·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Annika BordeJoshua Tobias
Oct 5, 2013·Clinical Microbiology Reviews·Matthew A CroxenB Brett Finlay
May 9, 2012·Physical Biology·Ling CaoXinghong Yang
Jan 1, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Charles H JonesBlaine A Pfeifer
Apr 5, 2018·Frontiers in Microbiology·Maricarmen Rojas-LopezRoberto Rosini
Oct 13, 2017·International Journal of Pharmaceutics·Stephanie LongetEd C Lavelle

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