PMID: 6411727Sep 25, 1983Paper

Construction of penP delta 1, Bacillus licheniformis 749/C beta-lactamase lacking site for lipoprotein modification. Expression in Escherichia coli and Bacillus subtilis.

The Journal of Biological Chemistry
P S MézesJ O Lampen

Abstract

Membrane-bound penicillinases in Gram-positive bacteria are glyceride-cysteine lipoproteins (Nielsen, J. B. K., and Lampen, J. O. (1982) J. Biol. Chem. 257, 4490-4495) that can be, but are not necessarily, intermediates in formation of exocellular enzymes. We have now deleted from the signal region of the Bacillus licheniformis 749/C beta-lactamase gene (penP) 15 base pairs that code for Ala-Leu-Ala-Gly-Cys. This sequence includes the Cys residue that undergoes lipophilic modification and the site of cleavage by signal peptidase and is well conserved in diverse prokaryotic lipoproteins. In the deletion gene, penP delta 1, the remaining Cys is preceded by 8 hydrophobic residues instead of 14 for the original modification site. PenP delta 1 has been cloned in Escherichia coli and Bacillus subtilis and its expression and products compared with penP. Penicillinase synthesis by penP delta 1 clones was greater than or equal to amounts formed by penP clones or B. licheniformis. Lipoprotein production from penP delta 1 was very low in either host and appears physiologically insignificant. In E. coli carrying penP delta 1, 25% of the penicillinase was released into the periplasm as a processed form. The remainder was a membrane-associat...Continue Reading

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.