Construction of pETNF-P16 plasmid and its expression properties in EC9706 cell line induced by X-ray irradiation

World Journal of Gastroenterology : WJG
Cong-Mei WuXiao-Hu Xu

Abstract

Recombined plasmid pETNF-P16 was constructed to investigate its expression properties in esophageal squamous carcinoma cell line EC9706 induced by X-ray irradiation and the feasibility of gene-radiotherapy for esophageal carcinoma. Recombined plasmid pETNF-P16 was constructed and transfected into EC9706 cells with lipofectamine. ELISA, Western blot, and immunocytochemistry were performed to determine the expression properties of pETNF-P16 in EC9706 after transfection induced by X-ray irradiation. Eukaryotic expression vector pETNF-P16 was successfully constructed and transfected into EC9706 cells. TNFalpha expressions were significantly increased in the transfected cells after different doses of X-ray irradiation than in those after 0Gy irradiation (1,192.330-2,026.518 pg/mL, P<0.05-0.01), and the TNFalpha expressions and P16 were significantly higher 6-48 h after 2 Gy X-ray irradiation (358.963-585.571 pg/mL, P<0.05-0.001). No P16 expression was detected in normal EC9706 cells. However, there was strong expression in the transfected and irradiation groups. X-ray irradiation induction could significantly enhance TNFalpha and P16 expression in EC9706 cells transfected with pETNF-P16 plasmid. These results may provide important e...Continue Reading

References

Jan 1, 1992·Immunologic Research·J RotheW Lesslauer
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B Christy, D Nathans
Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·R DattaD W Kufe
Mar 27, 1998·The New England Journal of Medicine·J MonzonN J Lassam
Jun 12, 1998·Biochemistry and Molecular Biology International·M ShimizuK Yagi
May 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·F GriscelliP Yeh
Jan 5, 2002·Acta Oncologica·R R WeichselbaumB Roizman
Feb 8, 2002·World Journal of Gastroenterology : WJG·An-Hui WangQing-Shu Chen
Jul 4, 2002·Canadian Journal of Gastroenterology = Journal Canadien De Gastroenterologie·Kenneth S WilsonGary J Dewar
Jul 12, 2002·Langenbeck's Archives of Surgery·Mitsuo TachibanaNaofumi Nagasue
Aug 2, 2002·American Journal of Clinical Oncology·Kenji NemotoShogo Yamada
Jan 18, 2003·World Journal of Gastroenterology : WJG·Xi-Jiang ZhaoQing-Lai Feng

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