PMID: 11311343Apr 20, 2001Paper

Construction of recombinant swinepox viruses and expression of the classical swine fever virus E2 protein

Journal of Virological Methods
J HahnB Y Ahn

Abstract

To explore the swinepox virus (SPV) as a potential live vector for immunization, a vector was developed for the construction of a recombinant SPV carrying foreign genes. In this system, a foreign gene placed under the strong vaccinia virus promoter P(11) can be inserted into the viral thymidine kinase (TK) gene, and the recombinant virus can be isolated in a non-selective medium by the co-expression of E. coli lacZ gene. Compared with the wild type virus, the TK(-)recombinant SPV showed a modest level of attenuation in porcine cells while more attenuation was observed in monkey or human cells. Using this system, a recombinant virus expressing the E2 glycoprotein of classical swine fever virus (CSFV) was produced. Engineered with the gX signal sequence of the pseudorabies virus, and transmembrane domain of E2, the E2 protein was expressed as a dimeric form in the cytoplasm of the infected cells.

References

Sep 11, 1987·Nucleic Acids Research·F G FalknerB Moss
Jan 1, 1966·Pathologia Veterinaria·N F Cheville
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·B Moss
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·E Paoletti

❮ Previous
Next ❯

Citations

Nov 6, 2003·Veterinary Microbiology·J T van Oirschot
Sep 18, 2003·Research in Veterinary Science·D J Paton, I Greiser-Wilke
Jun 30, 2005·Animal Health Research Reviews·Irene Greiser-Wilke, Volker Moennig
Jan 22, 2015·Animal Health Research Reviews·Vishal ChanderRishendra Verma
Jan 24, 2007·Vaccine·Martin BeerKlaus Depner
Aug 15, 2003·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·S TerzićJ Forsek
Jan 11, 2017·Veterinary Microbiology·Sandra BlomeMartin Beer
May 13, 2010·Archives of Biochemistry and Biophysics·Raquel MontesinoJosé A Cremata

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.