PMID: 6413824Jan 1, 1983Paper

Construction of recombination-deficient strains of Pseudomonas aeruginosa

Molecular & General Genetics : MGG
R FrühD Haas

Abstract

The rec-102 mutation had pleiotropic effects in Pseudomonas aeruginosa: low recombinational proficiency in conjugation and transduction; high UV sensitivity; inability to induce pyocin R2 by mitomycin C; and increased susceptibility to mitomycin C and nalidixic acid. The rec-102 locus was mapped by R68.45-mediated conjugation in the 45 min region of the PAO chromosome, between argF and thr-9001. By selection for a marker in this region, rec-102 can be introduced into a P. aeruginosa strain of interest using an R68.45 rec-102 donor. The recombination-deficient strains constructed in this way were phenotypically similar to Escherichia coli recA mutants.

References

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Mar 1, 1978·Journal of Bacteriology·B W Holloway
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Citations

Jul 1, 1987·Molecular & General Genetics : MGG·Y Sano, M Kageyama
Nov 15, 1983·Experientia·D Haas
Jul 16, 2014·Journal of Bacteriology·Jon PentermanGraham C Walker
Jan 1, 1990·Critical Reviews in Biochemistry and Molecular Biology·A I Roca, M M Cox
Apr 1, 1996·Microbiology·Saranga P KidambiRobert V Miller
Aug 1, 1988·Applied and Environmental Microbiology·S B O'MorchoeR V Miller
Dec 1, 1984·Journal of Bacteriology·F J Genthner, J D Wall
Aug 1, 1985·Journal of Bacteriology·T A Kokjohn, R V Miller
Feb 1, 1986·Journal of Bacteriology·M M Cavenagh, R V Miller
Feb 1, 1988·Journal of Bacteriology·T A Kokjohn, R V Miller

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