PMID: 9444992Jan 28, 1998Paper

Construction of retroviral vectors with improved safety, gene expression, and versatility

Journal of Virology
S H KimS Kim

Abstract

Murine leukemia virus (MLV)-based retroviral vectors are the most frequently used gene delivery vehicles. However, the current vectors are still not fully optimized for gene expression and viral titer, and many genetic and biochemical features of MLV-based vectors are poorly understood. We have previously reported that the retroviral vector MFG, where the gene of interest is expressed as a spliced mRNA, is superior in the level of gene expression with respect to other vectors compared in the study. As one approach to developing improved retroviral vectors, we have systematically performed mutational analysis of the MFG retroviral vector. We demonstrated that the entire gag coding sequence, together with the immediate upstream region, could be deleted without significantly affecting viral packaging or gene expression. To our knowledge, this region is included in all currently available retroviral vectors. In addition, almost the entire U3 region could be replaced with the heterologous human cytomegalovirus immediately-early promoter without deleterious effects. We could also insert internal ribosome entry sites (IRES) and multicloning sites into MFG without adverse effects. Based on these observations, we have constructed a seri...Continue Reading

References

Jan 1, 1987·Molecular and Cellular Biology·R B DuBridgeM P Calos
May 1, 1987·Journal of Virology·D ArmentanoE Gilboa
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·F HilbergM Grez
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J PriceC Cepko
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W R Osborne, A D Miller
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·O Danos, R C Mulligan
Aug 1, 1986·Molecular and Cellular Biology·A D Miller, C Buttimore
Oct 15, 1981·Nature·T M ShinnickJ G Sutcliffe
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·I RivièreR C Mulligan
Aug 25, 1995·Science·E Marshall
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·W S PearD Baltimore
Nov 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·G BandaraC H Evans
May 1, 1996·Applied Microbiology and Biotechnology·S G LeeB G Kim

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Citations

Jun 5, 2004·Annals of Medicine·Richard B ThompsonWalter J Koch
Jul 16, 2004·Journal of Virology·Masayuki KobayashiTakashi Uchiyama
Jan 8, 2003·The Journal of Gene Medicine·Youngtae HongSunyoung Kim
Jan 26, 2021·Frontiers in Oncology·Jiaqiao FanJianxun Song
May 18, 2000·Reviews in Medical Virology·G PalùM Pizzato
Aug 25, 2006·Biomaterials·Jennifer E PhillipsAndrés J García

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