Construction of shRNA lentiviral vector.

North American Journal of Medical Sciences
Hong Song, Ping-Chang Yang

Abstract

Lentiviruses have been adapted as gene delivery vehicles. This article summarized shRNA lentiviral vector methods generally used in research laboratories. The main procedures of shRNA lentiviral vector include that (1) Target sequences screening and shRNA oligonucleotides designing, (2) insert designed oligonucleotides into lentiviral vectors, (3) using packaging cells to produce shRNA lentivirus, and (4) transducing target cells with shRNA lentivirus.

Citations

Sep 13, 2012·Journal of Drug Delivery·Betzaida CastilloGabriel Barletta
Dec 2, 2015·Molecular Therapy. Nucleic Acids·Julia F AltermanAnastasia Khvorova
Jun 19, 2012·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·T LiuP-C Yang
Aug 29, 2013·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Jianhong WuYue Liu
Jun 23, 2018·Oncology Letters·Afiah Nasuha AznanZakiah Jubri
Jan 19, 2019·Genes & Genomics·Seong Kyun ParkByung Joon Hwang
Jun 26, 2018·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Qian DingZhuYun Huang
May 13, 2020·Biology Methods and Protocols·Anna Chiara PironaJörg D Hoheisel

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Methods Mentioned

BETA
restriction digest
transfection
FACS

Software Mentioned

DICER

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