Construction of streptavidin-luciferase fusion protein for ATP sensing with fixed form

Biotechnology Letters
Makiko NakamuraE Kobatake

Abstract

A fusion protein consisting of streptavidin and firefly luciferase was constructed to establish an accurate measuring technique of local ATP concentration. The fusion protein retained the binding ability of streptavidin and enzymatic activity of luciferase. Also, it could detect the concentration of antigens and could determine nanomolar concentrations of ATP in its fixed form via interactions with biotin-conjugated antibodies.

Citations

Mar 11, 2011·Bioscience, Biotechnology, and Biochemistry·Satoshi InouyeYuiko Sahara
Mar 28, 2006·Analytical Biochemistry·Makiko NakamuraEiry Kobatake
Nov 26, 2015·PloS One·Roy CohenAlexander J Travis
Nov 1, 2016·Photochemistry and Photobiology·Daria V Smirnova, Natalia N Ugarova
Nov 20, 2016·Photochemistry and Photobiology·Daria V SmirnovaNatalia N Ugarova
Apr 14, 2011·Chemical Society Reviews·Maureen A Walling, Jason R E Shepard
Dec 5, 2013·Biomacromolecules·Elizabeth M Wurtzler, David Wendell

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