journal cover

Construction, packaging and titration of recombinant adeno-associated virus vectors contaning antitumor genes

Zhonghua yi xue za zhi

Jul 23, 2002

Youbin ZhangJiayu Du

PMID: 12133507

Download from

PubMed

Abstract

To construct, package, and titrate vectors that express antitumor genes using adeno-associated virus (AAV) containing human alpha-fetoprotein (AFP) promoter. Recombinant AAV vectors were constructed by blunted ligation of plasmids rAAV-AFP-GFP and pTR-UF5 and p16 cDNA and p21 cDNA. Pack...read more

Mentioned in this Paper

Titrimetry
Shuttle Vectors
Oncoprotein p21
Virion
Cyclin-Dependent Kinase Inhibitor p16
Science of Morphology
Adeno-Associated Virus
Cosmids
Liver Carcinoma
Recombination, Genetic
Paper Details
References
  • References
  • Citations
  • finger pointing at paper

    References currently unavailable

    We're still populating references for this paper, please check back later.
  • References
  • Citations
  • quote and clock

    No citations available

    This paper may not have been cited yet.

Feeds With Similar Papers

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.

Cell-Type Specific Viral Vectors

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.

Cell-Type-Specific Viral Vectors (ASM)

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.

Cell-Type-Specific Viral Vectors

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.

Cyclins & Cyclin-dependent Kinases

Cyclins and cylin-dependent kinases (CDKs) are two classes of regulatory molecules that determine a cell’s progression through cell cycle. Here is the latest research.

AAV-based Gene Therapy

Adeno-associated virus (AAV)-based gene therapy is a biological vector that is being researched to be used as a potential therapeutic option. This gene therapy is designed to insert fragments of DNA into targeted cells to help treat diseases, such as hemophilia a. Discover the latest research on AAV-based gene therapy here.

Related Papers

Journal of Virology

Novel strategy for generation and titration of recombinant adeno-associated virus vectors

Journal of VirologyDecember 15, 2004
Ai-Li ShiauChao-Liang Wu
Laboratory Investigation; a Journal of Technical Methods and Pathology

Simple assay system for detecting human T cell leukemia virus type I-binding cells and its application in titrating binding inhibitory antibodies

Laboratory Investigation; a Journal of Technical Methods and PathologyNovember 1, 1993
H AkariS Harada
© 2020 Meta ULC. All rights reserved

Construction, packaging and titration of recombinant adeno-associated virus vectors contaning antitumor genes

Zhonghua yi xue za zhi

Jul 23, 2002

Youbin ZhangJiayu Du

PMID: 12133507

DOI:

Abstract

To construct, package, and titrate vectors that express antitumor genes using adeno-associated virus (AAV) containing human alpha-fetoprotein (AFP) promoter. Recombinant AAV vectors were constructed by blunted ligation of plasmids rAAV-AFP-GFP and pTR-UF5 and p16 cDNA and p21 cDNA. Pack...read more

Mentioned in this Paper

Titrimetry
Shuttle Vectors
Oncoprotein p21
Virion
Cyclin-Dependent Kinase Inhibitor p16
Science of Morphology
Adeno-Associated Virus
Cosmids
Liver Carcinoma
Recombination, Genetic

Feeds With Similar Papers

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.

Cell-Type Specific Viral Vectors

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.

Related Papers

Journal of Virology

Novel strategy for generation and titration of recombinant adeno-associated virus vectors

Journal of VirologyDecember 15, 2004
Ai-Li ShiauChao-Liang Wu
Laboratory Investigation; a Journal of Technical Methods and Pathology

Simple assay system for detecting human T cell leukemia virus type I-binding cells and its application in titrating binding inhibitory antibodies

Laboratory Investigation; a Journal of Technical Methods and PathologyNovember 1, 1993
H AkariS Harada
Paper Details
References
  • References
  • Citations
  • finger pointing at paper

    References currently unavailable

    We're still populating references for this paper, please check back later.
  • References
  • Citations
  • quote and clock

    No citations available

    This paper may not have been cited yet.

Download from

PubMed
/papers/construction-packaging-and-titration-of/12133507