PMID: 2505255Sep 1, 1989Paper

Construction, stable transformation, and function of an amber suppressor tRNA gene in Drosophila melanogaster

Proceedings of the National Academy of Sciences of the United States of America
F A LaskiG M Rubin

Abstract

Drosophila melanogaster strains with a stably incorporated amber suppressor tRNA gene have been generated. A tRNATyr gene was site specifically mutated to produce an anticodon sequence that recognizes the amber codon and then introduced into Drosophila by using P-element-mediated transformation. Transformants from four integration events were recovered. Two integrations resulted in both male and female sterility, whereas the other two resulted in male sterility but female fertility. Strains derived from the two female-fertile integration events were shown to have a low level of amber-suppressing activity by their ability to suppress an amber mutation in a chloramphenicol acetyltransferase gene.

References

Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A RazinA D Riggs
May 1, 1989·Genes & Development·M C MullinsG M Rubin
Jan 1, 1986·Cold Spring Harbor Symposia on Quantitative Biology·Y S HoY W Kan
Jan 1, 1985·Cold Spring Harbor Symposia on Quantitative Biology·W M GelbartR Grimaila
Aug 1, 1984·Cell·R E Karess, G M Rubin
Aug 26, 1983·Science·J F YoungP Palese
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·F A LaskiP A Sharp

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Citations

Jul 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J W SentryK Kaiser
Jul 1, 1993·Plant Molecular Biology·V T CarneiroI Small
Oct 1, 1993·Molecular & General Genetics : MGG·D B Pilgrim, J B Bell
Apr 10, 2004·Cancer Letters·Hiroko OhgakiMichael O Kurrer
Apr 20, 2005·Cancer Letters·Julien MazieresDavid M Jablons
Sep 17, 2013·Pattern Recognition·Amin ZollanvariEdward R Dougherty
Jan 1, 1997·Surgical Oncology·J R Howe, K C Conlon
Aug 7, 2012·Nature Chemical Biology·Ambra BiancoJason W Chin
Apr 11, 2000·Molecular and Cellular Biology·M BuvoliL A Leinwand
Apr 30, 2014·Annual Review of Genomics and Human Genetics·Kim M KeelingDavid M Bedwell
Apr 29, 2015·Proceedings of the National Academy of Sciences of the United States of America·Arpita BhattacharyaUttam L RajBhandary
Jan 7, 2010·Protein Science : a Publication of the Protein Society·Takahito MukaiShigeyuki Yokoyama
Jun 10, 2016·Nature·Joshua A ArribereAndrew Z Fire
Jul 22, 1998·Molecular and Cellular Biology·H J Park, U L RajBhandary
Aug 20, 2002·The Journal of Biological Chemistry·Joshua A Silverman, Pehr B Harbury
Feb 20, 2019·Nature Communications·John D LueckChristopher A Ahern
Aug 21, 2018·ACS Chemical Biology·Wes BrownAlexander Deiters
Sep 6, 2012·Cold Spring Harbor Protocols·Sean T SweeneyHaig Keshishian

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