Consumption of a high energy density diet triggers microbiota dysbiosis, hepatic lipidosis, and microglia activation in the nucleus of the solitary tract in rats.

Nutrition & Diabetes
Dulce MinayaKrzysztof Czaja

Abstract

Obesity is a multifactorial chronic inflammatory disease. Consumption of high energy density (HED) diets is associated with hyperphagia, increased body weight and body fat accumulation, and obesity. Our lab has previously shown that short-term (4 weeks) consumption of a HED diet triggers gut microbiota dysbiosis, gut inflammation, and reorganization of the gut-brain vagal communication. The aim of this study was to investigate the effect of long-term (6 months) consumption of HED diet on body composition, gut microbiome, hepatocellular lipidosis, microglia activation in the nucleus of the solitary tract, and systemic inflammation. Male Sprague-Dawley rats were fed a low energy density (LED) diet for 2 weeks and then switched to a HED diet for 26 weeks. Twenty-four-hour food intake, body weight, and body composition were measured twice a week. Blood serum and fecal samples were collected at baseline, 1, 4, 8, and 26 weeks after introduction of the HED diet. Serum samples were used to measure insulin, leptin, and inflammatory cytokines using Enzyme-linked Immunosorbent Assay. Fecal samples were assessed for 16 S rRNA genome sequencing. HED diet induced microbiota dysbiosis within a week of introducing the diet. In addition, there...Continue Reading

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Citations

Jul 30, 2020·Current Opinion in Pharmacology·Liliana Espinoza, Carie R Boychuk
Mar 12, 2021·Advances in Nutrition·Kirsten BerdingJohn F Cryan
May 18, 2021·The Journal of Clinical Investigation·Kristie B Yu, Elaine Y Hsiao

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Methods Mentioned

BETA
ELISA

Software Mentioned

cellSense Standard
Olympus
METAGENassist
Nikon Elements AR
Quantitative Insights Into Microbial Ecology ( QIIME )
GraphPad Prism

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