Contact allergens and irritants show discrete differences in the activation of human monocyte-derived dendritic cells: consequences for in vitro detection of contact allergens

Archives of Toxicology
Frank StraubePeter Ulrich

Abstract

In recent years test systems have been described that may be applied routinely to discriminate between contact allergens and irritants in vitro. Using human monocyte-derived dendritic cells (MoDC), this study was designed to refine the settings of a potential routine screening protocol for contact allergens and to investigate the so far poorly defined concentration dependency of contact allergen-specific effects. MoDC were generated by 6 days of culture in the presence of IL-4 and GM-CSF and were then cultured for 24 or 48 h in medium with lipopolysaccharide (LPS), contact allergens [picrylsulphonic acid (TNBS), 1-chloro-2,4-dinitrobenzene (DNCB)] or irritants [sodium dodecyl sulphate (SDS), benzalkonium chloride (BAC)] that were free of detectable endotoxin contamination. The induction of CD86 and HLA-DR expression was quantified by flow cytometry as markers for MoDC activation. LPS activation upregulated CD86 about 20-fold and HLA-DR expression about 4-fold. Compared to LPS, contact allergens had weaker effects. TNBS and DNCB induced activation marker upregulation starting slightly below the cytotoxic concentration and increasing in a dose-dependent manner. However, at partially cytotoxic concentrations, irritants also induce...Continue Reading

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Citations

Jul 13, 2011·Journal of Allergy·Stacey E AndersonB J Meade
Nov 26, 2015·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Xi YuMin Hong
Feb 20, 2007·Toxicology and Applied Pharmacology·François PythonPierre Aeby
May 24, 2008·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·N J HoldenS C Knight
Jan 25, 2014·Journal of Immunological Methods·Anna KoryakinaPeter Bruegger
Jul 15, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Cindy A RyanIan Kimber
Nov 17, 2006·The Journal of Pharmacology and Experimental Therapeutics·Eve M CoulterDean J Naisbitt
Oct 30, 2008·Journal of Immunotoxicology·Nadege AdeFrancoise Rousset
Jan 7, 2020·Alternatives to Laboratory Animals : ATLA·Bianca MariglianiAnna Maria Buehler
Jan 8, 2009·Chemical Research in Toxicology·Hagen OttAnn-Therese Karlberg

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