Contact bubble bilayers with flush drainage

Scientific Reports
Masayuki Iwamoto, Shigetoshi Oiki

Abstract

Planar lipid bilayers have been used to form stable bilayers into which membrane proteins are reconstituted for measurements of their function under an applied membrane potential. Recently, a lipid bilayer membrane is formed by the apposition of two monolayers that line an oil-electrolyte interface. Here, a bilayer membrane system is developed with picoliter bubbles under mechanically and chemically manipulable conditions. A water bubble lined with a phospholipid monolayer is blown from a glass pipette into an oil phase. Two blowing pipettes are manipulated, and bubbles (each with a diameter of ~ 50 μm) are held side by side to form a bilayer, which is termed a contact bubble bilayer. With the electrode implemented in the blowing pipette, currents through the bilayer are readily measured. The intra-bubble pressure is varied with the pressure-controller, leading to various sizes of the bubble and the membrane area. A rapid solution exchange system is developed by introducing additional pressure-driven injection pipettes, and the blowing pipette works as a drain. The solution is exchanged within 20 ms. Also, an asymmetric membrane with different lipid composition of each leaflet is readily formed. Example applications of this ver...Continue Reading

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Citations

Mar 10, 2016·Journal of the American Chemical Society·Yuka MatsukiShigetoshi Oiki
Mar 7, 2018·Proceedings of the National Academy of Sciences of the United States of America·Rikiya WatanabeShigekazu Nagata
Apr 1, 2018·The Journal of Biological Chemistry·Seiji KojimaHideyuki Takahashi
Mar 1, 2018·The Journal of General Physiology·Laura-Marie WintersteinGerhard Thiel
Oct 13, 2018·Chemical Communications : Chem Comm·Maie A ElfaramawyTomoaki Matsuura
Jan 17, 2020·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Maki KomiyaAyumi Hirano-Iwata
Aug 2, 2017·Scientific Reports·Masayuki Iwamoto, Shigetoshi Oiki
Sep 9, 2017·Scientific Reports·Masayuki IwamotoShigetoshi Oiki
Dec 5, 2018·Proceedings of the National Academy of Sciences of the United States of America·Masayuki Iwamoto, Shigetoshi Oiki
Mar 2, 2018·Biological & Pharmaceutical Bulletin·Shigetoshi Oiki, Masayuki Iwamoto
Jan 14, 2021·The Journal of General Physiology·Laura-Marie WintersteinOliver Rauh
Mar 31, 2019·Biochimica Et Biophysica Acta. General Subjects·Rikiya Watanabe
Mar 21, 2021·Proceedings of the National Academy of Sciences of the United States of America·Kenichiro MitaShigetoshi Oiki
Sep 24, 2021·Biophysical Journal·Mahroof KalathingalShinji Saito

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Methods Mentioned

BETA
fluorescence imaging

Software Mentioned

pCLAMP
ImageJ

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