Contact-dependent cell interactions determine hormone responsiveness and desensitization in rat granulosa cells

Endocrinology
F Harandian, R Farookhi

Abstract

The maintenance of associations between granulosa cells (GCs) is necessary for FSH-stimulated induction of LH receptors. In cultures in which these associations have been disrupted, FSH fails to induce LH receptors. As FSH exerts its action in GCs via cAMP, we have examined if the aggregation state of GCs plays a role in modulating FSH-stimulated cAMP production. GCs were obtained from the ovaries of diethylstilbestrol-primed immature rats. Cells were prepared as aggregate or dispersed populations by isolating GCs in either the presence or absence of Ca2+. Nonviable cells were removed by a brief exposure to trypsin. We have shown previously that trypsin treatment in the absence of Ca2+ removes a class of cell adhesion molecules, termed cadherins, from the plasma membranes of GCs. Hence, the dispersed GCs are incapable of reaggregating. Dispersed or aggregate GC preparations were incubated with different doses of human FSH (0-1 microg) for 0-60 min in the presence of isobutylmethylxanthine, a phosphodiesterase inhibitor. Incubations were terminated, and the cAMP accumulated was measured using a specific RIA. As desensitization to hormonal stimuli is a characteristic property of many G protein-coupled response systems, cAMP produ...Continue Reading

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Citations

Jul 2, 2005·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Rikako KawagishiYuji Murata
Jan 23, 2002·Molecular Reproduction and Development·Naomi H MachellRiaz Farookhi
Aug 23, 2000·Biology of Reproduction·N H MachellR Farookhi

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