PMID: 3768379Nov 7, 1986Paper

Contact inhibition within hemoglobin S polymer by thiol reagents

Biochimica Et Biophysica Acta
J Caburi-MartinY Beuzard

Abstract

N-Ethylmaleimide, a thiol reagent, increases the solubility of deoxyhemoglobin S. We investigated which of the two reacted beta 93 cysteine residues of the Hb tetramer was responsible for the inhibition of Hb S polymerization. Accordingly we compared the solubility of equal mixtures of HbA + HbS, HbA NEM + HbS and HbA + HbS NEM. Upon deoxygenation these mixtures contain about 50% a stable and asymmetrical hybrid alpha 2A beta A beta S, alpha 2A beta A,NEM beta S or alpha 2A beta A beta S,NEM respectively and 25% parental molecules as confirmed by ion-exchange HPLC performed in anaerobic conditions. Within the hybrid molecule, beta A or beta A,NEM chain has to be present in the alpha beta dimer located in trans to the dimer which contains the only beta 6 valine residue participating in intermolecular contacts (dimer in cis), while beta S or beta S,NEM must be in cis position in the hybrid molecule. The solubility of mixtures increases 4% for HbA NEM + HbS and 20% for HbA + HbS NEM mixtures compared to HbA + HbS mixture, indicating that the inhibitory effect of N-ethylmaleimide is more effective in cis than in trans position. The absence of a major role played by N-ethylmaleimide located in trans was supported by the solubility s...Continue Reading

References

Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R L NagelK Matsutomo
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Aug 22, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Guillaume SandozFlorian Lesage

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Citations

Jan 22, 2010·European Journal of Applied Physiology·Anna BogdanovaKatja Heinicke
Jan 1, 2009·Indian Journal of Clinical Biochemistry : IJCB·Murali WoodiKrishnaswamy Patnam Rajagopalan
Mar 14, 2007·Biophysical Chemistry·Kelly M KneeIshita Mukerji
Aug 18, 1995·The Journal of Biological Chemistry·M J RaoA S Acharya
Nov 15, 1990·Biochimica Et Biophysica Acta·M C GarelY Beuzard

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