Contact-mediated inhibition of human haematopoietic progenitor cell proliferation may be conferred by stem cell antigen, CD34

The Hematology Journal : the Official Journal of the European Haematology Association
M Y GordonJ M Goldman

Abstract

The function of CD34, a transmembrane sialomucin expressed by human haematopoietic progenitor cells, is poorly understood. Its structure suggests it may act as a cell adhesion and signalling molecule. KGIa cells and primary CD34-positive marrow cells were tested for their ability to aggregate in the presence of the anti-CD34 antibody QBEND10; CFU-GM colonies were grown using standard methods and tested for their content of colony-forming cells by replating; 'haematons' were isolated from marrow by filtration; the phosphorylation of CD34 was investigated by immunoprecipitation and Western blotting CD34-positive cells in human bone marrow, like KG1a cells, aggregate when incubated with QBEND10. Staining aggregates with anti-CD34-FITC revealed that aggregation involved co-localisation of CD34 at intercellular binding sites. We examined myeloid colonies (CFU-GM) grown from normal human bone marrow cells, and multicellular aggregates ('haematons') separated from freshly aspirated marrow by filtration, and found CD34-positive cells bound together with co-localisation of the CD34 at the binding sites. This finding shows that CD34-positive cell-cell adhesion occurs physiologically in vitro and in vivo. QBEND10-induced aggregation of KG...Continue Reading

Citations

Aug 22, 2003·British Journal of Haematology·Karen A WhitingRuth Pettengell
Oct 24, 2002·Blood·Hanna S RadomskaClaudia S Huettner
Nov 14, 2008·Stem Cells and Development·Moubarak MouiseddineJean-Marc Bertho
Nov 5, 2005·Journal of Biomaterials Science. Polymer Edition·Evelyn K F Yim, Kam W Leong
Nov 25, 2005·Stem Cells and Development·Gurudutta U GangenahalliPratibha M Luthra
Jul 19, 2006·Stem Cells and Development·Gurudutta U GangenahalliPratibha M Luthra
May 25, 2007·Stem Cells and Development·Claudia LangeBoris Fehse

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