Contact-mediated intracellular delivery of hydrophobic drugs from polymeric nanoparticles

Cancer Nanotechnology
Sofie SnipstadCatharina de Lange Davies

Abstract

Encapsulation of drugs in nanoparticles can enhance the accumulation of drugs in tumours, reduce toxicity toward healthy tissue, and improve pharmacokinetics compared to administration of free drug. To achieve efficient delivery and release of drugs at the target site, mechanisms of interaction between the nanoparticles and cells and the mechanism of delivery of the encapsulated drug are crucial to understand. Our aim was to determine the mechanisms for cellular uptake of a fluorescent hydrophobic model drug from poly(butylcyanoacrylate) nanoparticles. Prostate adenocarcinoma cells were incubated with Nile Red-loaded nanoparticles or free Nile Red. Uptake and intracellular distribution were evaluated by flow cytometry and confocal laser scanning microscopy. The nanoparticles mediated a higher intracellular level and more rapid uptake of encapsulated Nile Red compared to model drug administered alone. The main mechanism for delivery was not by endocytosis of nanoparticles but by nanoparticle-cell contact-mediated transfer directly to the cytosol and, to a smaller extent, release of payload from nanoparticles into the medium followed by diffusion into cells. The payload thus avoids entering the endocytic pathway, evading lysosoma...Continue Reading

References

May 1, 1992·Histochemistry·W J BrownP Greenspan
Mar 1, 1985·The Journal of Cell Biology·P GreenspanS D Fowler
Jan 1, 1994·Cancer Chemotherapy and Pharmacology·A Colin de VerdièreP Couvreur
Aug 3, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jayanth PanyamVinod Labhasetwar
Mar 12, 2003·Advanced Drug Delivery Reviews·Jayanth Panyam, Vinod Labhasetwar
Apr 23, 2003·Advanced Drug Delivery Reviews·Christine VauthierPatrick Couvreur
Mar 17, 2004·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Alexander BootzJörg Kreuter
Nov 25, 2005·Current Drug Delivery·Jayanth Panyam, Vinod Labhasetwar
Jul 11, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Patrick M WinterGregory M Lanza
Jul 13, 2006·Annual Review of Biomedical Engineering·Vladimir P Torchilin
Feb 13, 2007·Nanomedicine : Nanotechnology, Biology, and Medicine·Catarina Pinto ReisFrancisco Veiga
Mar 11, 2008·Micron : the International Research and Review Journal for Microscopy·Giacomo DiazAngela Maria Falchi
Jul 26, 2008·Nature Nanotechnology·Dan PeerRobert Langer
Aug 14, 2008·Macromolecular Bioscience·Julia DausendVolker Mailänder
Nov 28, 2008·Molecular Pharmaceutics·Peisheng XuYoon Yeo
Feb 24, 2009·Biomaterials·Malgorzata S CartieraW Mark Saltzman
Apr 1, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Hae Yun NamSeo Young Jeong
Jun 6, 2009·Cellular and Molecular Life Sciences : CMLS·Hervé Hillaireau, Patrick Couvreur
Sep 29, 2009·Colloids and Surfaces. B, Biointerfaces·Avnesh KumariSubhash C Yadav
Jan 6, 2010·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Julien Nicolas, Patrick Couvreur
Jan 13, 2010·Molecular Pharmaceutics·Silvia PetersenHeike Bunjes
May 8, 2010·Chemical Communications : Chem Comm·Davide BrambillaPatrick Couvreur
Jun 15, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·You Han Bae, Kinam Park
Jul 2, 2011·Nanomedicine·Jesse V JokerstSanjiv S Gambhir
Jul 8, 2011·Accounts of Chemical Research·Xiaowei MaXing-Jie Liang
Oct 15, 2011·PloS One·Tiziana TenutaIseult Lynch
Mar 6, 2012·Chemical Society Reviews·Nazila KamalyOmid C Farokhzad
Jul 18, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sebastien TaurinKhaled Greish
Sep 6, 2012·International Journal of Biological Macromolecules·Zuzana GaraiovaCatharina de L Davies
Apr 30, 2013·Pharmaceutical Research·Qiaoya LinGang Zheng
Dec 19, 2013·Chemical Communications : Chem Comm·Daniel HofmannVolker Mailänder
Oct 29, 2012·RSC Advances·Andrey S KlymchenkoThierry F Vandamme

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Citations

Feb 14, 2020·Critical Reviews in Toxicology·Asaad AzarnezhadAmirhossein Ahmadi
Jan 9, 2016·Journal of Nanobiotechnology·Einar SulheimCatharina de Lange Davies
Nov 22, 2017·International Journal of Molecular Sciences·Einar SulheimÝrr Mørch
Feb 12, 2021·Expert Opinion on Drug Delivery·Roghayeh SheervalilouHabib Ghaznavi
Feb 8, 2021·Ultrasound in Medicine & Biology·Sofie SnipstadSigrid Berg
Aug 21, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Mahmuda NargisYasuhito Koyama
May 13, 2020·Pharmaceutical Research·Baptiste MartinSophie Franceschi
Dec 13, 2019·ACS Applied Materials & Interfaces·Eun Young HwangDong Woo Lim
Nov 1, 2016·Bioconjugate Chemistry·Ashley V KrollLiangfang Zhang

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Methods Mentioned

BETA
flow cytometry
dynamic light scattering
FRET
transmission microscopy
Fluorescence
fluorescence imaging

Software Mentioned

Excel
Kaluza Flow Cytometry Analysis
Amira
SigmaPlot

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