Contemporary techniques for freezing mouse spermatozoa

Current Protocols in Mouse Biology
Mo GuanMartin Fray

Abstract

Each year, thousands of new mouse models are generated around the world to further biomedical research. Unfortunately, the cost of maintaining mouse colonies makes it uneconomical to keep strains on the shelf that are not part of active research programs. Ideally, these retired strains should be archived. If this is not done and the line is simply killed off, the genetics are lost to future generations of scientists. Traditionally, embryo freezing has been used to cryopreserve mice, but this is expensive, time consuming, requires large numbers of donor females, and usually involves invasive superovulation procedures. Sperm freezing circumvents all of these disadvantages and is rapidly becoming the technique of choice for many repositories. This has been made possible through the use of refined cryoprotective agents and the development of improved in vitro fertilization techniques. This article describes two popular sperm freezing techniques employed by mouse repositories to archive spermatozoa using cryoprotective agents supplemented with either L-glutamine or monothioglycerol.

References

Feb 1, 1986·Journal of in Vitro Fertilization and Embryo Transfer : IVF·P H Glenister, M F Lyon
Jul 25, 2000·Mammalian Genome : Official Journal of the International Mammalian Genome Society·P H Glenister, C E Thornton
Nov 25, 2000·Biology of Reproduction·J M SzteinL E Mobraaten
Apr 25, 2008·Current Protocols in Immunology·John Donovan, Patricia Brown
Oct 14, 2009·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Dominika D PetersMartin Hrabé de Angelis
Sep 4, 2012·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Steve D M Brown, Mark W Moore
Feb 28, 2015·Current Protocols in Mouse Biology·Mo GuanMartin Fray

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Citations

Jun 6, 2016·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Michael RaessUNKNOWN INFRAFRONTIER Consortium
Mar 4, 2020·Scientific Reports·Ming-Wen Li, K C Kent Lloyd
Jun 22, 2018·Current Protocols in Mouse Biology·Andrew BleasePaul K Potter
Sep 24, 2020·Laboratory animal research·Toru TakeoNaomi Nakagata
Aug 21, 2018·Theriogenology·Marcello RaspaFerdinando Scavizzi

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