Continual cell deformation induced via attachment to oriented fibers enhances fibroblast cell migration

PloS One
Sisi QinMiriam H Rafailovich

Abstract

Fibroblast migration is critical to the wound healing process. In vivo, migration occurs on fibrillar substrates, and previous observations have shown that a significant time lag exists before the onset of granulation tissue. We therefore conducted a series of experiments to understand the impact of both fibrillar morphology and migration time. Substrate topography was first shown to have a profound influence. Fibroblasts preferentially attach to fibrillar surfaces, and orient their cytoplasm for maximal contact with the fiber edge. In the case of en-mass cell migration out of an agarose droplet, fibroblasts on flat surfaces emerged with an enhanced velocity, v = 52μm/h, that decreases to the single cell value, v = 28μm/h within 24 hours and remained constant for at least four days. Fibroblasts emerging on fibrillar surfaces emerged with the single cell velocity, which remained constant for the first 24 hours and then increased reaching a plateau with more than twice the initial velocity within the next three days. The focal adhesions were distributed uniformly in cells on flat surfaces, while on the fibrillar surface they were clustered along the cell periphery. Furthermore, the number of focal adhesions for the cells on the f...Continue Reading

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Citations

Feb 20, 2016·Current Opinion in Cell Biology·Alexandra Lynn McGregorJan Lammerding
Nov 4, 2016·Journal of Biomedical Materials Research. Part a·Nathaniel J HogrebeKeith J Gooch
Feb 2, 2017·Beilstein Journal of Nanotechnology·Alexandra M GreinerRalf Kemkemer
Jul 3, 2019·Npj Regenerative Medicine·Thomas Lee Jenkins, Dianne Little

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nuclear translocation

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GraphPad Prism

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