Continued Activity of the Pioneer Factor Zelda Is Required to Drive Zygotic Genome Activation

Molecular Cell
Stephen L McDanielMelissa M Harrison

Abstract

Reprogramming cell fate during the first stages of embryogenesis requires that transcriptional activators gain access to the genome and remodel the zygotic transcriptome. Nonetheless, it is not clear whether the continued activity of these pioneering factors is required throughout zygotic genome activation or whether they are only required early to establish cis-regulatory regions. To address this question, we developed an optogenetic strategy to rapidly and reversibly inactivate the master regulator of genome activation in Drosophila, Zelda. Using this strategy, we demonstrate that continued Zelda activity is required throughout genome activation. We show that Zelda binds DNA in the context of nucleosomes and suggest that this allows Zelda to occupy the genome despite the rapid division cycles in the early embryo. These data identify a powerful strategy to inactivate transcription factor function during development and suggest that reprogramming in the embryo may require specific, continuous pioneering functions to activate the genome.

Citations

Aug 24, 2019·Nature Structural & Molecular Biology·John T Lis
Dec 5, 2019·Briefings in Functional Genomics·Charlotte MorettiYad Ghavi-Helm
May 30, 2020·Genes & Development·Jihyun IrizarryAngelike Stathopoulos
Oct 24, 2019·Development·Daniel KruegerStefano De Renzis
Oct 30, 2020·Frontiers in Cell and Developmental Biology·Mingtian DengFeng Wang
Sep 5, 2020·Annual Review of Genetics·Kenneth S Zaret
Feb 3, 2021·Cellular and Molecular Life Sciences : CMLS·Darya ChetverinaPaul Schedl
Feb 10, 2021·G3 : Genes - Genomes - Genetics·Miles W DeAngelisRuth I Johnson
Jul 5, 2019·Bio-protocol·Stephen L McDaniel, Melissa M Harrison
Mar 11, 2021·Molecular Cell·Elizabeth D LarsonMelissa M Harrison
Jun 27, 2021·Seminars in Cell & Developmental Biology·Alphy John, Matteo Rauzi
Jul 14, 2021·Current Opinion in Structural Biology·Wataru Kobayashi, Kikuë Tachibana
Mar 17, 2021·Annual Review of Biomedical Engineering·Payam E FarahaniJared E Toettcher
Aug 17, 2021·The EMBO Journal·Ranjith ViswanathanStefano De Renzis
Sep 1, 2021·Metabolomics : Official Journal of the Metabolomic Society·Jhenifer Nascimento da SilvaCarlos Logullo

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