Continuous 2-keto-L-gulonic acid fermentation from L-sorbose by Ketogulonigenium vulgare DSM 4025

Applied Microbiology and Biotechnology
Yoshinori TakagiTatsuo Hoshino

Abstract

A single-stage continuous fermentation process for the production of 2-keto-L-gulonic acid (2KGA) from L-sorbose using Ketogulonigenium vulgare DSM 4025 was developed. The chemostat culture with the dilution rate that was calculated based on the relationship between the 2KGA production rate and the 2KGA concentration was feasible for production with high concentration of 2KGA. In this system, 112.2 g/L of 2KGA on the average was continuously produced from 114 g/L of L-sorbose. A steady state of the fermentation was maintained for the duration of more than 110 h. The dilution rate was kept in the range of 0.035 and 0.043 h(-1), and the 2KGA productivity was 3.90 to 4.80 g/L/h. The average molar conversion yield of 2KGA from L-sorbose was 91.3%. Under the optimal conditions, L-sorbose concentration was kept at 0 g/L. Meanwhile, the dissolved oxygen level was changing in response to the dilution rate and 2KGA concentration. In the dissolved oxygen (DO) range of 16% to 58%, it was revealed that the relationship between DO and D possessed high degree of positive correlation under the L-sorbose limiting condition (complete consumption of L-sorbose). Increasing D closer to the critical value for washing out point of the continuous fer...Continue Reading

References

Mar 1, 1987·Journal of Medical Ethics·A R Dyer, S Bloch
Jan 1, 1970·Pathologia et microbiologia·P Krieg, L Ettlinger
Feb 15, 1984·The Biochemical Journal·S F Leong, J B Clark
May 1, 1982·Applied and Environmental Microbiology·T SonoyamaK Mitsushima
Jun 1, 2006·Biotechnology and Bioengineering·M JeudeJ Büchs

❮ Previous
Next ❯

Citations

Aug 10, 2012·Applied Microbiology and Biotechnology·Mervi H ToivariMarilyn G Wiebe
Apr 24, 2014·Critical Reviews in Biotechnology·Saúl AlonsoMario Díaz
Feb 7, 2021·Biotechnology Advances·Zhengshan LuoJingwen Zhou

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.