Continuous affinity-gradient nano-stationary phase served as a column for reversed-phase electrochromatography and matrix carrier in time-of-flight mass spectrometry for protein analysis

Analytica Chimica Acta
Jen-Kuei WuFan-Gang Tseng

Abstract

This study developed an affinity-gradient nano-stationary phase (AG-NSP) for protein analysis using nanofluidic capillary electrochromatography (nano-CEC) conjugated with matrix assisted laser desorption ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS). The AG-NSP can be used for protein pre-separation in nano-CEC and as a matrix carrier for protein analysis in MALDI-TOF-MS. A hydrophobicity gradient in AG-NSP was photochemically formed by grafting 4-azidoaniline hydrochloride on vertically arrayed multi-wall carbon nanotubes (MWCNTs) through gray-level exposure to UV light. The reversed-phase gradient stationary phase in AG-NSP was tailored according to the properties of the mobile phase gradient in capillary electrochromatography. As a result, the operation of the system is easily automated using a single buffer solution without the need for multiple solvents for elution. The use of nano-CEC with AG-NSP demonstrated excellent separation efficiency and high resolution for various types of DNA/protein/peptide. MALDI-TOF-MS analysis was then performed directly on the separated proteins and peptides on the chip. The proposed system was then used for the detection of three types of proteins with different molecular w...Continue Reading

References

Jan 27, 2007·Electrophoresis·Ivo Nischang, Ulrich Tallarek
Aug 15, 2009·Lab on a Chip·Samuel Tia, Amy E Herr
Jul 7, 2010·Electrophoresis·Karine Faure
May 9, 2012·Lab on a Chip·Klaus B Mogensen, Jörg P Kutter

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Citations

May 21, 2016·Analytical Chemistry·Veeren C DewoolkarMaryanne M Collinson
Nov 3, 2016·Journal of Separation Science·Sille Štěpánová, Václav Kašička
Nov 4, 2016·Journal of Separation Science·Ivan Mikšík
Feb 9, 2017·Journal of Pharmaceutical and Biomedical Analysis·Xiaoya JiangHongyuan Yan

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