Continuous annular chromatography

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Frank Hilbrig, Ruth Freitag

Abstract

The principle of continuous annular chromatography (CAC) has been known for several decades. CAC is a continuous chromatographic mode, which lends itself to the separation of multi-component mixtures as well as of bi-component ones. In CAC, the mobile and stationary phases move in a crosscurrent fashion, which allows transformation of the typical one-dimensional batch column separation into a continuous two-dimensional one. With the exception of linear gradient elution, all chromatographic modes have at present been applied in CAC. This review focuses on the capacity of CAC for preparative bioseparation. The historical developments and the predecessors of modern CAC are briefly summarized. The state-of-the-art in the theoretical prediction and simulation of CAC separations is discussed, followed by an overview of current CAC instrumentation and example applications, especially for the isolation of proteins and other bio(macro)molecules. In this context, issues of scale up as well as method development and transfer from batch to continuous CAC columns are discussed using recent bioseparation efforts as pertinent examples.

References

Aug 5, 1969·Journal of Chromatography·R A Nicholas, J B Fox
Feb 28, 1997·Journal of Chromatography. a·K ReissnerC H Byers
Feb 15, 2001·Biotechnology Progress·A BuchacherD Josic
Feb 24, 2001·Journal of Chromatography. a·A UretschlägerA Jungbauer
Jan 12, 2002·Biotechnology and Bioengineering·R Giovannini, R Freitag
Sep 25, 2003·Bioprocess and Biosystems Engineering·Andrea Uretschläger, Alois Jungbauer

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Citations

Feb 26, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xindu Geng, Lili Wang
Sep 25, 2009·Biotechnology Journal·Roland Wohlgemuth
Jul 5, 2016·Biotechnology Journal·Fabian SteinebachMassimo Morbidelli
Feb 13, 2015·Preparative Biochemistry & Biotechnology·Anurag S RathoreS Muthukumar
Dec 16, 2016·Biotechnology Progress·Nikhil KatejaAnurag S Rathore
Jul 14, 2021·Analytical Chemistry·Sven KochmannSergey N Krylov
Jul 4, 2018·Analytical Chemistry·Sven Kochmann, Sergey N Krylov

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