Continuous antibiotic treatment for experimental abdominal sepsis: effects on organ inflammatory cytokine expression and neutrophil sequestration

The British Journal of Surgery
M A Mercer-JonesW G Cheadle

Abstract

Tumour necrosis factor (TNF) alpha and interleukin (IL) 1 beta are produced in the lung after peritonitis and may contribute to neutrophil-mediated organ injury. It was hypothesized that, during experimental peritonitis, continuous rather than intermittent antibiotic therapy would reduce lung expression of TNF-alpha and IL-1 beta messenger RNA (mRNA) and neutrophil sequestration. After caecal ligation and puncture, mice received either intermittent or continuous cefoxitin, or continuous metronidazole or aztreonam. Cytokine mRNAs were determined by reverse transcription differential polymerase chain reaction and lung neutrophil content by myeloperoxidase (MPO) assay. Continuous cefoxitin reduced median (interquartile range (i.q.r.)) lung IL-1 beta mRNA expression ((ratio to beta-actin): continuous 0.18 (0.14-0.34), intermittent 0.46 (0.44-0.49), saline 0.43 (0.38-0.53), P < 0.05) and median (i.q.r.) lung MPO content (continuous 22.5 (9.7-40), intermittent 65 (57.5-76), saline 47 (41-64), P < 0.05) compared with intermittent therapy and saline controls. Continuous infusion was also associated with reduced bacteraemia (P < 0.05) but not serum TNF-alpha or endotoxin levels. Both continuous metronidazole and aztreonam reduced lung M...Continue Reading

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Citations

Feb 21, 2003·Surgical Infections·Todd WaltripSusan Galandiuk
Jul 20, 2007·Surgical Infections·Dustin R WoodsWilliam G Cheadle
Oct 26, 2010·Surgical Infections·Apostolos G KambaroudisThomas Gerasimidis
Aug 31, 2000·Critical Care Medicine·M HeinzelmannW G Cheadle
Feb 6, 2004·Critical Care Medicine·Kevin C DoerschugGary W Hunninghake
Jul 13, 2004·European Journal of Haematology·E KnudsenP O Iversen
Mar 22, 2007·European Journal of Clinical Investigation·M Odeh
Nov 25, 2000·The Journal of International Medical Research·K TammeR Talvik
Jun 7, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Xinchun LinEdmund J Miller
Jun 26, 2007·Shock·J W Olivier van TillMarja A Boermeester

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