PMID: 9438349Jan 1, 1997Paper

Continuous cultivation of bakers' yeast: change in cell composition at different dilution rates and effect of heat stress on trehalose level

Folia Microbiologica
N Ertugay, H Hamamci

Abstract

The cell composition of bakers' yeast in a continuous culture was determined for different dilution rates. Also, the cellular response to heat stress in terms of trehalose, RNA, glycogen and protein was determined at a specified dilution rate of 0.1/h. The amount of storage saccharides, trehalose and glycogen, was found to decrease whereas the amount of RNA and protein increased with increasing dilution rates. As the dilution rate was increased from 0.1 to 0.4/h at 0.05 intervals the steady-state trehalose content decreased from 33 to 8.6 mg/g biomass, and glycogen content from 150 to 93 mg/g biomass. On the other hand, the protein content increased from 420 to 530 mg/g biomass and the RNA content from 93 to 113 mg/g biomass. Heat stress was applied by increasing the medium temperature from 30 to 36, 38 or 40 degrees C at constant dilution rates. The highest amount of trehalose accumulation, 108 mg/g biomass, was achieved when heat stress at 38 degrees C was applied. The protein content, on the other hand, decreased from 350 to 325 mg/g biomass at the end of the experiment.

References

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Citations

Dec 4, 2003·Journal of Theoretical Biology·Fernando Alvarez-VasquezEberhard O Voit
Dec 20, 2008·Canadian Journal of Microbiology·Radoslav I AbrashevMaria B Angelova
Jan 10, 2009·Biotechnology and Bioengineering·Arthur M A PistoriusTatjana A Egorova-Zachernyuk
May 15, 2013·Molecular Systems Biology·Yi-Fan XuJoshua D Rabinowitz
Dec 25, 2015·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·A G ZebitayE Vuruşkan
Aug 14, 2019·Proceedings of the National Academy of Sciences of the United States of America·Yu Chen, Jens Nielsen

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