Continuous culture of Cryptosporidium parvum using hollow fiber technology

International Journal for Parasitology
Mary MoradaNigel Yarlett

Abstract

Diarrheal disease is a leading cause of pediatric death in economically low resource countries. Cryptosporidium spp. are the second largest member of this group and the only member for which no treatment exists. One of the handicaps to developing chemotherapy is the lack of a reproducible long-term culture method permitting in vitro drug screening beyond 48 h. We have adapted the well-established hollow fiber technology to provide an environment that mimics the gut by delivering nutrients and oxygen from the basal layer upwards while allowing separate redox and nutrient control of the lumen for parasite development. Using this technique, oocyst production was maintained for >6 months, producing approximately 1×10(8)oocysts ml(-1)day(-1), compared with 48 h with a yield of 1×10(6)oocysts ml(-1) in two-dimensional cultures. Oocysts, after 4 and 20 weeks in culture, produced a chronic infection in a TCR-α-deficient mouse model. In vivo infectivity of oocysts was confirmed using oocysts from a 6 week culture in a dexamethasone immunosuppressed mouse model.

References

Sep 1, 1997·Molecular and Biochemical Parasitology·J S KeithlyN Yarlett
Mar 27, 2004·Science·Mitchell S AbrahamsenVivek Kapur
Jan 25, 2005·The Pediatric Infectious Disease Journal·Yaghoob HamediMehregan Haidari
Aug 24, 2007·Eukaryotic Cell·Jolly Mazumdar, Boris Striepen
Oct 16, 2007·Applied and Environmental Microbiology·Laura Y Sifuentes, George D Di Giovanni
Dec 25, 2007·Free Radical Biology & Medicine·Melissa KempDean P Jones
Apr 11, 2009·Experimental Parasitology·Lihua Xiao
Oct 1, 2010·Parasitology·M YichoyS Das
Aug 13, 2011·Free Radical Research·Magdalena L Circu, Tak Yee Aw
May 15, 2012·Lancet·Li LiuUNKNOWN Child Health Epidemiology Reference Group of WHO and UNICEF
Aug 11, 2012·International Journal for Parasitology·J NiemandK Kirk
Mar 20, 2013·Infection and Immunity·Alejandro Castellanos-GonzalezA Clinton White
Aug 30, 2013·The Journal of Biological Chemistry·Mary MoradaNigel Yarlett
Oct 4, 2013·Science Translational Medicine·Rahul P BakshiTheresa A Shapiro
Nov 16, 2013·Nature·Boris Striepen
Jun 4, 2014·Molecular and Biochemical Parasitology·Nimisha MittalRentala Madhubala
Jul 30, 2014·Journal of Microbiological Methods·Eunice A VarugheseJagjit S Yadav
Oct 4, 2014·The Lancet Infectious Diseases·William CheckleyEric R Houpt

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Citations

Sep 15, 2016·ACS Infectious Diseases·Ujjini H ManjunathaThierry T Diagana
Jul 28, 2016·Parasite Immunology·U RyanA Paparini
Aug 12, 2017·Current Protocols in Microbiology·Mattie C PawlowicBoris Striepen
Nov 17, 2017·Parasitology·Panagiotis Karanis
Nov 17, 2017·Clinical Microbiology Reviews·Lynne S GarciaGovinda Visvesvara
Jul 24, 2018·Human Vaccines & Immunotherapeutics·Sangun LeeSaul Tzipori
Aug 14, 2018·Advanced Healthcare Materials·Clara R CorreiaJoão F Mano
Jan 6, 2017·Infection and Immunity·Maria A DeCicco RePassHonorine D Ward
Dec 23, 2016·Journal of Clinical Microbiology·Per SikoraRomanico B G Arrighi
Feb 9, 2019·Current Protocols in Microbiology·Lyne JosséAnastasios D Tsaousis
May 28, 2019·Scientific Reports·Lucas V S MatosGiovanni Widmer
Jul 4, 2019·Microbiology·Rachel M ChalmersKevin Tyler
Mar 21, 2019·The Journal of Infectious Diseases·Sangun LeeSaul Tzipori
Sep 8, 2020·Expert Opinion on Drug Discovery·Melissa S Love, Case W McNamara
Jun 28, 2018·Nature Microbiology·Inha HeoHans Clevers
Apr 25, 2019·Nature Microbiology·Jessica C Kissinger
Nov 3, 2017·The Journal of Parasitology·A Paziewska-HarrisH D F H Schallig
Sep 22, 2017·Frontiers in Microbiology·Yaoyu Feng, Lihua Xiao
Apr 16, 2019·Gut Pathogens·Christopher N MillerAnastasios D Tsaousis
Jan 18, 2017·Database : the Journal of Biological Databases and Curation·Olukemi O IfeonuJoana C Silva
Sep 20, 2018·F1000Research·Seema BhalchandraHonorine D Ward
Apr 26, 2018·PLoS Neglected Tropical Diseases·Geneva GraefJennifer A Zambriski
Mar 14, 2018·Parasites & Vectors·Marzieh Ezzaty MirhashemiGiovanni Widmer
Jul 28, 2019·Trends in Parasitology·Sonja RueckertAnastasios D Tsaousis
Feb 2, 2021·Frontiers in Cellular and Infection Microbiology·Yiran LiJessica C Kissinger
Jan 28, 2020·Trends in Parasitology·Elisabeth A InnesMattie C Pawlowic
Jun 12, 2021·Frontiers in Cellular and Infection Microbiology·Charles K Crawford, Amir Kol
Jul 3, 2021·Genes·Rodrigo P BaptistaJessica C Kissinger
Oct 16, 2019·ACS Infectious Diseases·Rajiv S JumaniUjjini H Manjunatha
Aug 5, 2018·Bio-protocol·Nigel Yarlett, Mary Morada
Aug 27, 2021·PLoS Neglected Tropical Diseases·Smita Sutrave, Martin Heinrich Richter
Sep 3, 2021·Food and Waterborne Parasitology·Jennifer K O'LearyBrigid Lucey
Aug 3, 2018··Mary MoradaNigel Yarlett

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