PMID: 37154Feb 1, 1979

Continuous culture of normal adult mammalian hepatocytes exhibiting parenchymal functions

In Vitro
H M VickreyD S McCann


Past in vitro studies of liver-cell functions have been performed on nonproliferating primary cells or serially propagated hepatic monolayers of neoplastic or fetal origin. We optimized conditions for the selective culture of adult rabbit and canine liver parenchymal cells and presently have four differentiated proliferating monolayer strains. At the 30th passage level these hepatic cultures still display the specific liver parenchymal functions of albumin and fibrinogen synthesis as well as tyrosine aminotransferase activity. Optimization of the conditions for hepatocyte culture was monitored by [3H]thymidine incorporation. Albumin and fibrinogen synthesis were measured by bioradioimmunoassay and tryosine transaminase activity by a modification of Diamondstone's assay. Albumin and fibrinogen synthesis were correlated with hepatocyte growth kinetics.


Apr 21, 1975·Biochemical and Biophysical Research Communications·J R Emanuel, T D Gelehrter
May 19, 1975·Biochemical and Biophysical Research Communications·R C Lin, P J Snodgrass
Oct 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·R A RichmanD L Friedman
Jan 1, 1969·Experimental Cell Research·J M VasilievR I Rappoport
Oct 1, 1956·The Journal of Experimental Medicine·S J CIECIURAT T PUCK


Sep 1, 1991·Digestive Diseases and Sciences·T TakahashiY Nosé
Feb 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·G K InnesK E Hobbs
Nov 1, 1982·The Anatomical Record·J E KlaunigB F Trump
Jan 1, 1980·Annals of the New York Academy of Sciences·L M ReidM Rojkind
Aug 24, 2010·The Psychoanalytic Quarterly·Eric Glassgold

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