Continuous culture of Reuber hepatoma cells in serum-free arginine-, glutamine- and tyrosine-deprived chemically defined medium

In Vitro
A NiwaY Yasumura

Abstract

The rat hepatoma cell line, H4-II-E, was grown serially over a 1-year period and about 30 passages in arginine-, glutamine-, and tyrosine-deprived and ornithine-supplemented Eagle's minimum essential medium with no supplements other than biotin. The adapted cell line, R-Y121B, proliferates in the above mentioned medium with a doubling time of about 4 days and maintains hepatic "marker" enzymes such as tyrosine aminotransferase, phenylalanine hydroxylase, and all the enzymes of the urea cycle.

Citations

Feb 1, 1993·Amino Acids·K Yamamoto, A Niwa
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·T NakamuraA Ichihara
Oct 5, 1988·Biotechnology and Bioengineering·M C GlassyP C Chau
Jul 1, 1984·The Journal of Pharmacy and Pharmacology·K SorimachiY Yasumura
Jul 18, 1984·Biochemical and Biophysical Research Communications·K Sorimachi, Y Yasumura
Mar 1, 1987·Somatic Cell and Molecular Genetics·F D LedleyS L Woo

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