Continuous cultures of macrophages derived from the 8-day epiblast of the pig

In Vitro Cellular & Developmental Biology. Animal
N C TalbotV G Pursel

Abstract

Secondary macrophage cell cultures were generated from the primary culture of epiblasts of 8-d-old pig blastocysts. The epiblast-derived macrophagelike (EDM) cells have a morphology and ameboid behavior that is typical of tissue histocytes. The cells reacted positively with monoclonal antibodies specific for pig granulocyte-macrophage lineage cells, and were not reactive with monoclonal antibodies specific for pig B and T lymphocytes. Marked phagocytic behavior and the formation of phagosomes were demonstrated following incubation with FITC-labeled bacteria. The EDM cells stained positively for nonspecific acid esterase that was not inhibited by sodium fluoride. DiI-acetylated-LDL was rapidly taken up by the cells. Transmission electron microscopy of the EDM cells showed phagolysosomes, numerous cytoplasmic vacuoles, large, lobed nuclei, and numerous pseudopods or filopodia at the cell surface. Strong reactivity of the cells with anti-CD14 monoclonal antibody was observed. Further, cytotoxic activity was produced from the EDM cells after exposure to lipopolysaccharide in a concentration and time-dependent manner. The cultures could be maintained and expanded for several months on STO co-culture. Their derivation from the epibla...Continue Reading

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Citations

Jul 14, 1998·Veterinary Immunology and Immunopathology·N C TalbotM Worku
Jan 17, 2008·The Journal of Reproduction and Development·Irena VackovaFederica Lopes
Jun 22, 2007·Theriogenology·T A L BreviniF Gandolfi
Oct 24, 2006·Theriogenology·Tiziana A L BreviniFulvio Gandolfi
Nov 24, 2012·The Journal of General Virology·Sophie RogéeNicole Pavio
Dec 21, 2005·Journal of Applied Animal Welfare Science : JAAWS·M C Appleby
Aug 29, 2002·In Vitro Cellular & Developmental Biology. Animal·Neil C TalbotWesley M Garrett

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