Continuous cytometric bead processing within a microfluidic device for bead based sensing platforms

Lab on a Chip
Sung YangJeffrey D Zahn

Abstract

A microfluidic device for continuous biosensing based on analyte binding with cytometric beads is introduced. The operating principle of the continuous biosensing is based on a novel concept named the "particle cross over" mechanism in microfluidic channels. By carefully designing the microfluidic network the beads are able to "cross-over" from a carrier fluid stream into a recipient fluid stream without mixing of the two streams and analyte dilution. After crossing over into the recipient stream, bead processing such as analyte-bead binding may occur. The microfluidic device is composed of a bead solution inlet, an analyte solution inlet, two washing solution inlets, and a fluorescence detection window. To achieve continuous particle cross over in microfluidic channels, each microfluidic channel is precisely designed to allow the particle cross over to occur by conducting a series of studies including an analogous electrical circuit study to find optimal fluidic resistances, an analytical determination of device dimensions, and a numerical simulation to verify microflow structures within the microfluidic channels. The functionality of the device was experimentally demonstrated using a commercially available fluorescent biotiny...Continue Reading

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Dec 3, 2005·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Sung YangJeffrey D Zahn
Jun 29, 2006·Lab on a Chip·Sung YangJeffrey D Zahn
Nov 23, 2006·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Sung YangJeffrey D Zahn
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Citations

Oct 24, 2013·Analytical and Bioanalytical Chemistry·Mark D TarnNicole Pamme
Jun 28, 2008·Lab on a Chip·Peter B HowellFrances S Ligler
Dec 22, 2009·Artificial Organs·Akif UndarJohn L Myers
Aug 10, 2010·Microfluidics and Nanofluidics·Lawrence A SassoJeffrey D Zahn
Jul 12, 2012·Lab on a Chip·Temsiri SongjaroenWanida Laiwattanapaisal
Sep 18, 2018·Electrophoresis·Suman Chakraborty
Dec 19, 2008·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Shigang WangAkif Undar
Dec 20, 2011·Lab on a Chip·Kwang W OhEdward P Furlani
Oct 1, 2012·Microfluidics and Nanofluidics·Lawrence A SassoJeffrey D Zahn

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