PMID: 9186285Jan 1, 1997Paper

Continuous detection of superoxide in situ during ischemia and reperfusion in the rabbit heart

Japanese Heart Journal
S UshirodaM Nakano

Abstract

In order to clarify the time course of superoxide generation in situ during ischemia and reperfusion in the rabbit heart, we used a method of enhanced chemiluminescence (CL) with 2-methyl-6-[p-methoxyphenyl]-3, 7-dihydroimidazo [1, 2-alpha]pyrazin-3-one (MCLA) as a specific probe for detecting superoxide radicals. The surface of the rabbit heart was exposed to a photomultiplier tube in a light-proof box. We introduced a reversible snare occluder into the box to continuously observe the light emission. An ischemia-reperfusion group (I/R, n = 7) was subjected to 30 mins of coronary occlusion, followed by 90 mins of reperfusion. We performed the same procedure (except for coronary occlusion) in the sham-operated group (n = 4). Another group of rabbits (n = 4) subjected to I/R received superoxide dismutase (SOD: 20 mg/kg, i.v.) during reperfusion to observe the CL response. In the I/R-group, the increase in CL began at 13 +/- 2 (mean +/- SEM) mins and peaked at 52 +/- 12 mins of reperfusion. CL in the I/R-group gradually increased from 818 +/- 350 counts/10 secs in the preischemic period to 1077 +/- 401 counts/10 secs during reperfusion (p < 0.01). In contrast, there was no increase in CL in the sham-operated group. The administrat...Continue Reading

Citations

Apr 1, 2000·Clinical and Experimental Pharmacology & Physiology·K YamashitaH Fujiwara
Mar 27, 2001·Japanese Circulation Journal·J MidorikawaY Maruyama
Sep 30, 2006·Bioorganic Chemistry·Katsunori Teranishi
Feb 6, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Margaret M TarpeyMatthew B Grisham
Nov 6, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Courtney L WilliamsonJohn M Hollander
Jan 25, 2000·American Journal of Physiology. Cell Physiology·A A OgonowskiR W Caldwell

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