Continuous directed evolution for strain and protein engineering

Current Opinion in Biotechnology
Simon d'Oelsnitz, Andrew Ellington

Abstract

Continuous directed evolution is the 'while loop' of synthetic biology, autonomous cycles of mutation, selection, and self-replication that can lead to the rapid development of industrially relevant organisms, pathways, or molecules. Although this engineering strategy requires particular mutagenesis methods and well-defined selections, recent advances have facilitated its implementation. Control over selection pressure has been augmented by novel cost-effective continuous culturing devices with open source designs. New in vivo targeted mutagenesis methods have enabled continuous directed protein evolution in various organisms. Furthermore, advances in automation have enabled rational, semi-continuous directed evolution strategies that may yield fewer artefacts or parasites. Overall, continuous directed evolution is persistently demonstrating its capacity to rapidly generate biotechnologically valuable strains and proteins.

References

May 28, 2019·Journal of Industrial Microbiology & Biotechnology·Sungho JangGyoo Yeol Jung
Sep 20, 2019·Journal of Industrial Microbiology & Biotechnology·Weizhu ZengJingwen Zhou
May 5, 2020·Natural Product Reports·Stephanie GalanieJames Lalonde
Jul 31, 2020·PLoS Biology·Harrison SteelAntonis Papachristodoulou
May 13, 2018·Genes·Denis ShepelinMarkus J Herrgård

Related Concepts

Directed Evolution
In Vivo
Biochemical Pathway
Virus Replication
Cell Culture Techniques
In Vitro Molecular Evolution
Mutagenesis Procedure
Laboratory Culture
Parasites
Protein Engineering

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