Continuous Formation of Ultrathin, Strong Collagen Sheets with Tunable Anisotropy and Compaction.

ACS Biomaterials Science & Engineering
Shashi MalladiAxel Günther

Abstract

The multiscale organization of protein-based fibrillar materials is a hallmark of many organs, but the recapitulation of hierarchal structures down to fibrillar scales, which is a requirement for withstanding physiological loading forces, has been challenging. We present a microfluidic strategy for the continuous, large-scale formation of strong, handleable, free-standing, multicentimeter-wide collagen sheets of unprecedented thinness through the application of hydrodynamic focusing with the simultaneous imposition of strain. Sheets as thin as 1.9 μm displayed tensile strengths of 0.5-2.7 MPa, Young's moduli of 3-36 MPa, and modulated the diffusion of molecules as a function of collagen nanoscale structure. Smooth muscle cells cultured on engineered sheets oriented in the direction of aligned collagen fibrils and generated coordinated vasomotor responses. The described biofabrication approach enables rapid formation of ultrathin collagen sheets that withstand physiologically relevant loads for applications in tissue engineering and regenerative medicine, as well as in organ-on-chip and biohybrid devices.

References

May 6, 1976·Cell and Tissue Research·R F Dyer, C D Enna
Mar 1, 1986·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·M J LevesqueR M Nerem
Jan 23, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·N L'HeureuxF A Auger
Aug 31, 2002·Biophysical Journal·Saroja RamanujanRakesh K Jain
Mar 15, 2003·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·A Ekani-Nkodo, D Kuchnir Fygenson
Apr 30, 1957·The Journal of Physiology·D M MAURICE
Aug 1, 1965·Biological Reviews of the Cambridge Philosophical Society·D H ELLIOTT
Feb 24, 2006·Nature Medicine·Nicolas L'HeureuxTodd N McAllister
Aug 10, 2006·Proceedings of the National Academy of Sciences of the United States of America·Markus J Buehler
Nov 23, 2006·Biomaterials·Cheng Guo, Laura J Kaufman
Jul 9, 2008·Biomaterials·Babette LanferCarsten Werner
Apr 5, 1994·Biotechnology and Bioengineering·J F CavallaroK H Kraus
Jul 9, 2009·Physiological Reviews·Jessica E Wagenseil, Robert P Mecham
Dec 22, 2009·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Jeffrey M CavesElliot L Chaikof
May 10, 2011·Biomaterials·Jeffrey M CavesElliot L Chaikof
Oct 14, 2011·Integrative Biology : Quantitative Biosciences From Nano to Macro·Patrick W AlfordKevin Kit Parker
Jun 21, 2012·Advanced Materials·Lian LengAxel Günther
Jun 8, 2013·Acta Biomaterialia·Vivek A KumarElliot L Chaikof
Dec 19, 2013·Biomaterials Science·Vivek A KumarElliot L Chaikof
Apr 18, 2014·Biophysical Journal·Jieling Zhu, Laura J Kaufman
Jun 26, 2014·Journal of Visualized Experiments : JoVE·Dominique TremblayAndrew E Pelling
Aug 12, 2013·Advanced Functional Materials·Ashutosh AgarwalKevin Kit Parker
Aug 12, 2016·Nano Letters·Christian HaynlThomas Scheibel
Nov 23, 2017·International Journal of Biological Macromolecules·Fatemeh AjalloueianIoannis S Chronakis
Apr 18, 2018·Lab on a Chip·Navid HakimiAxel Günther
Jul 18, 2019·Biofabrication·David J BarrettRuth E Cameron
Jan 14, 2019·ACS Biomaterials Science & Engineering·Jaejung SonJe-Kyun Park
Oct 10, 2016·ACS Biomaterials Science & Engineering·Stephanie RheeLawrence J Bonassar

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Citations

Mar 12, 2021·Advanced Healthcare Materials·Wuyang GaoAxel Günther
Apr 1, 2021·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Constantine TarabanisElliot L Chaikof
Apr 15, 2021·American Journal of Physiology. Cell Physiology·Adeel AhmedVinay V Abhyankar
Jun 22, 2021·Advanced Materials Technologies·Adeel AhmedVinay V Abhyankar
Sep 17, 2020·ACS Applied Materials & Interfaces·Danilo M Dos SantosLuiz H C Mattoso

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