Continuous high expression of XBP1 and GRP78 is important for the survival of bone marrow cells in CCl4-treated cirrhotic liver

Biochemical and Biophysical Research Communications
Yoshio MarumotoIsao Sakaida

Abstract

We have previously shown that infusion of bone marrow cells (BMC) improves CCl(4)-induced cirrhosis. However, it is unclear why the injected BMC are resistant to CCl(4) damage and subsequently improve the local microenvironment in damaged liver. To analyze the cellular phenomena involved in this process, we studied the damaged liver using electron microscopy. We found that CCl(4) caused rough endoplasmic reticula to swell in hepatocytes. To analyze the gene expression patterns associated with this process, we conducted PCR-selected suppressive subtractive hybridization. We found that expression levels of HSP84, HSP40, and XBP1 differed markedly between control liver and liver infused with BMC. Immunohistochemical staining revealed that expression levels of HSP84 and HSP40 were markedly higher in the early phase of differentiation immediately after BMC infusion, but decreased over time. XBP1 expression remained high during the late phase, and GRP78 expression increased with XBP1 activation. We also found that GFP-positive BMC expressed XBP1 and GRP78. XBP1 and GRP78 are associated with ER stress. Thus, continuous high XBP1 and GRP78 expression might be essential for the survival and proliferation of BMC in a CCl(4)-induced persi...Continue Reading

References

Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·L DiatchenkoP D Siebert
Dec 29, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·N D TheiseD S Krause
Jun 28, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·N D TheiseD S Krause
Aug 5, 2000·Nature·M R AlisonN A Wright
Apr 5, 2002·Nature·Qi-Long YingAustin G Smith
Sep 21, 2002·Lancet·Eriko Tateishi-YuyamaUNKNOWN Therapeutic Angiogenesis using Cell Transplantation (TACT) Study Investigators
Mar 18, 2003·The Journal of Clinical Investigation·Andreea IanusMehboob A Hussain
Jan 2, 2004·Nature Medicine·Takao IwawakiMasayuki Miura
Nov 27, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Isao SakaidaKiwamu Okita
May 18, 2005·The Journal of Cell Biology·Keiko NakanishiNobuhiro Morishima
Jun 20, 2006·Biochemical and Biophysical Research Communications·Takeshi FunakoshiYoichi Mizukami

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Jan 29, 2010·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Hongyang ShiLi Zhang
Sep 30, 2009·European Journal of Pharmacology·Hongyang ShiLi Zhang
Jan 27, 2011·Ultrastructural Pathology·Neriman ColakogluMustafa Sarsilmaz

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