Continuous Laminaribiose Production Using an Immobilized Bienzymatic System in a Packed Bed Reactor

Applied Biochemistry and Biotechnology
Akram AbiHans-Joachim Jördening

Abstract

The first continuous production system of laminaribiose from sucrose and glucose in a bienzymatic reaction is reported in this study. Immobilized laminaribiose phosphorylase and sucrose phosphorylase were used in a packed bed reactor system comprising of a 3-cm glass column at 35 °C with a steady feeding flow rate of 0.1 ml/min. Factors affecting product formation including enzyme ratio, peal concept (both enzymes in one pearl or in separate pearls), and pearl size were studied. An enzyme ratio of 2:1 of laminaribiose phosphorylase (LP) to sucrose phosphorylase (SP) when encapsulated separately in bigger size peals resulted in higher concentration of product. Laminaribiose (0.4 g/(L h)) is produced in the optimized system at steady state. The reaction system proved to be operationally stable throughout 10 days of continuous processing. A half-life time of more than 9 days was observed for both biocatalysts.

References

Jul 1, 1992·Journal of Biochemistry·M KitaokaH Taniguchi
Nov 1, 2005·Journal of Biomedical Materials Research. Part a·Hyojin ParkDukjoon Kim
Jan 1, 2014·International Journal of Biological Macromolecules·Yu OgawaMasahisa Wada
Jun 12, 2017·Bioprocess and Biosystems Engineering·Clarissa MüllerHans-Joachim Jördening

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Citations

Nov 5, 2019·Biotechnology Journal·Chao Zhong, Bernd Nidetzky
Oct 4, 2018·Engineering in Life Sciences·Akram AbiHans-Joachim Jördening
May 11, 2021·Applied Microbiology and Biotechnology·Zorica UbiparipTom Desmet
Aug 16, 2021·Carbohydrate Research·Gregory S BulmerJolanda M van Munster

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