Continuous monitoring of intracellular volumes in isolated rat hearts during normothermic perfusion and ischemia

Journal of Magnetic Resonance
N Askenasy, G Navon

Abstract

The present study describes an experimental setup that enables continuous measurement of cellular volumes in isolated organs. The procedure is a modification of a recently reported method that uses multinuclear NMR measured by 59Co NMR of cobalticyanide and 1H NMR of water in isolated rat hearts at normothermia. The new apparatus contains a background flow which is shown to improve the rate of exchange of the marker between the interstitium and the external solution and allows detection of cellular shrinkage during no-flow ischemia. A series of experiments of marker loading and wash-out were performed to validate the method. In the Langendorff preparation, intracellular volumes (in units of milliliters per gram dry weight) of hearts perfused with Krebs-Henseleit solution oscillated around a mean value of 2.50 +/- 0.06 ml/gdw. During 30 min of ischemia the cells swelled to 2.88 +/- 0.08 ml/gdw and residual edema was observed after 30 min of reperfusion (2.62 +/- 0.08 ml/gdw). A hypoosmotic shock was used to assess changes in membrane permeability at different time points of ischemia and reperfusion. Water influx induced by the hypoosmotic shock at the end of ischemia was similar to that elicited in perfused hearts. After 15 and ...Continue Reading

Citations

Mar 29, 2011·Cardiovascular Research·Sergey MissanTerence F McDonald
Jul 11, 2012·Basic Research in Cardiology·Arkady RutkovskiyJarle Vaage
Oct 26, 2013·Basic Research in Cardiology·Arkady RutkovskiyJarle Vaage
Dec 15, 2012·Journal of Molecular and Cellular Cardiology·Arkady RutkovskiyKåre-Olav Stensløkken
Jan 18, 2003·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·K BendjelidM Janier
Apr 6, 2004·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Govindasamy IlangovanPeriannan Kuppusamy
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Mar 26, 2005·Canadian Journal of Physiology and Pharmacology·R SouthworthP B Garlick
Apr 19, 2013·Journal of Cardiovascular Pharmacology·Emiliano R DiezRoberto M Miatello
Feb 6, 2020·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Hala AraibiRoisin Kelly-Laubscher
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