Continuous non-cell autonomous reprogramming to generate retinal ganglion cells for glaucomatous neuropathy

Stem Cells
Sowmya ParameswaranIqbal Ahmad

Abstract

Glaucoma, where the retinal ganglion cells (RGCs) carrying the visual signals from the retina to the visual centers in the brain are progressively lost, is the most common cause of irreversible blindness. The management approaches, whether surgical, pharmacological, or neuroprotective do not reverse the degenerative changes. The stem cell approach to replace dead RGCs is a viable option but currently faces several barriers, such as the lack of a renewable, safe, and ethical source of RGCs that are functional and could establish contacts with bona fide targets. To address these barriers, we have derived RGCs from the easily accessible adult limbal cells, reprogrammed to pluripotency by a non-nucleic acid approach, thus circumventing the risk of insertional mutagenesis. The generation of RGCs from the induced pluripotent stem (iPS) cells, also accomplished non-cell autonomously, recapitulated the developmental mechanism, ensuring the predictability and stability of the acquired phenotype, comparable to that of native RGCs at biochemical, molecular, and functional levels. More importantly, the induced RGCs expressed axonal guidance molecules and demonstrated the potential to establish contacts with specific targets. Furthermore, w...Continue Reading

References

Apr 1, 1987·The Journal of Physiology·S A Lipton, D L Tauck
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G R Martin
Jan 1, 1997·Experimental Eye Research·J C MorrisonE C Johnson
May 1, 1997·Developmental Biology·I AhmadD L Polk
Nov 30, 1999·Lancet·A L Coleman
Sep 23, 2000·Science·C J Neumann, C Nuesslein-Volhard
Aug 29, 2003·Development·Michael E ZuberWilliam A Harris
Oct 24, 2003·Nature·L M CalviD T Scadden
Apr 14, 2004·Archives of Ophthalmology·Leonard A LevinTeresa Borrás
May 28, 2004·The Journal of Comparative Neurology·David H RapaportMatthew M LaVail
Apr 6, 2005·Developmental Cell·Juan-Ramon Martinez-MoralesJoachim Wittbrodt
Feb 27, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Patricio A RiquelmeFiona Doetsch
Jun 15, 2007·Developmental Biology·Lynda Erskine, Eloisa Herrera
Sep 29, 2007·Brain Research·Rajesh K Sharma, Peter A Netland
Mar 29, 2008·Cell Stem Cell·Shihuan KuangMichael A Rudnicki
Apr 22, 2009·Neuroscience Letters·Yasuhiko HiramiMasayo Takahashi
Jul 7, 2009·Annual Review of Cell and Developmental Biology·Michalis Agathocleous, William A Harris
May 21, 2010·Investigative Ophthalmology & Visual Science·Mengfei ChenJian Ge
Apr 22, 2011·Cellular and Molecular Life Sciences : CMLS·Trevor M YeungCalvin J Kuo
Aug 6, 2011·Cell Stem Cell·Bonnie Barrilleaux, Paul S Knoepfler
Sep 4, 2012·Current Opinion in Pharmacology·Thomas V Johnson, Keith R Martin
Jan 17, 2013·Current Gene Therapy·Alona O Cramer, Robert E MacLaren
Apr 20, 2013·Stem Cells Translational Medicine·David E BuchholzDennis O Clegg
May 30, 2013·Development·Kazutoshi Takahashi, Shinya Yamanaka
Feb 5, 2014·Stem Cells Translational Medicine·Hamidreza RiazifarTaosheng Huang

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Citations

Dec 10, 2015·Stem Cells International·Louise A Mesentier-LouroMarcelo F Santiago
Oct 28, 2016·Progress in Retinal and Eye Research·Susanna S ParkJan A Nolta
Oct 9, 2018·Frontiers in Neuroscience·Oriane RabesandratanaGaël Orieux
Dec 18, 2019·Progress in Retinal and Eye Research·Iqbal AhmadXiaohuan Xia
Jan 23, 2021·Genes·Marta García-LópezM Esther Gallardo

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