Continuous particle separation using pressure-driven flow-induced miniaturizing free-flow electrophoresis (PDF-induced μ-FFE)

Scientific Reports
Hyungkook JeonGeunbae Lim

Abstract

In this paper, we introduce pressure-driven flow-induced miniaturizing free-flow electrophoresis (PDF-induced μ-FFE), a novel continuous separation method. In our separation system, the external flow and electric field are applied to particles, such that particle movement is affected by pressure-driven flow, electroosmosis, and electrophoresis. We then analyzed the hydrodynamic drag force and electrophoretic force applied to the particles in opposite directions. Based on this analysis, micro- and nano-sized particles were separated according to their electrophoretic mobilities with high separation efficiency. Because the separation can be achieved in a simple T-shaped microchannel, without the use of internal electrodes, it offers the advantages of low-cost, simple device fabrication and bubble-free operation, compared with conventional μ-FFE methods. Therefore, we expect the proposed separation method to have a wide range of filtering/separation applications in biochemical analysis.

References

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Citations

Apr 18, 2018·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Bram BetRené van Roij
Jan 1, 2018·Microfluidics and Nanofluidics·Bram BetSela Samin
Mar 9, 2019·Physical Review Letters·A SłapikJ Spiechowicz
Nov 11, 2016·Lab on a Chip·Thoriq SalafiYong Zhang
Dec 1, 2020·ACS Nano·Yuliang XieTony Jun Huang
Mar 3, 2020·ACS Omega·Suhyeon KimGeunbae Lim

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Methods Mentioned

BETA
chip
electrophoresis

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